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Literature summary extracted from

  • Grebenok, R.J.; Galbraith, D.W.; Benveniste, I.; Feyereisen, R.
    Ecdysone 20-monooxygenase , a cytochrome P-450 enzyme from spinach, Spinacia oleracea (1996), Phytochemistry, 42, 927-933.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.14.99.22 instable, linear activity under standard assay conditions for 10 min Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.99.22 CO partially reversible by irradiation with monochromatic light Spinacia oleracea
1.14.99.22 additional information in pure N2 atmosphere, enzyme activity is 50% inhibited due to missing oxygen Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.99.22 0.025
-
ecdysone
-
Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.99.22 microsome
-
Spinacia oleracea
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.22 ecdysone + NADPH + O2 Spinacia oleracea
-
20-hydroxyecdysone + NADP+ + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.22 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.22 not: purification of the enzyme from microsomes failed due to unstability of the enzyme Spinacia oleracea

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.99.22 ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein Spinacia oleracea
1.14.99.22 ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O ecdysteroid biosynthesis regulation Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.99.22 leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.99.22 0.0021
-
microsomes Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
1.14.99.22 -80°C, quick frozen membranes, 15% glycerol v/v, at least 1 month Spinacia oleracea
1.14.99.22 4°C to -20°C storage for prolonged periods not possible due to unstability of the enzyme, appreciable loss of activity Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.22 alpha-ecdysone + NADPH + O2
-
Spinacia oleracea 20-hydroxyecdysone + NADP+ + H2O
-
?
1.14.99.22 ecdysone + NADPH + O2
-
Spinacia oleracea 20-hydroxyecdysone + NADP+ + H2O
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.22 cytochrome P450 essential Spinacia oleracea
1.14.99.22 NADPH ultimate hydrogen donor, cannot be substituted by NADH or ascorbate Spinacia oleracea