EC Number | General Stability | Organism |
---|---|---|
1.14.99.22 | instable, linear activity under standard assay conditions for 10 min | Spinacia oleracea |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.99.22 | CO | partially reversible by irradiation with monochromatic light | Spinacia oleracea | |
1.14.99.22 | additional information | in pure N2 atmosphere, enzyme activity is 50% inhibited due to missing oxygen | Spinacia oleracea |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.99.22 | 0.025 | - |
ecdysone | - |
Spinacia oleracea |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.99.22 | microsome | - |
Spinacia oleracea | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.22 | ecdysone + NADPH + O2 | Spinacia oleracea | - |
20-hydroxyecdysone + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.99.22 | Spinacia oleracea | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.99.22 | not: purification of the enzyme from microsomes failed due to unstability of the enzyme | Spinacia oleracea |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.99.22 | ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O | NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein | Spinacia oleracea | |
1.14.99.22 | ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O | ecdysteroid biosynthesis regulation | Spinacia oleracea |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.99.22 | leaf | - |
Spinacia oleracea | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.99.22 | 0.0021 | - |
microsomes | Spinacia oleracea |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.99.22 | -80°C, quick frozen membranes, 15% glycerol v/v, at least 1 month | Spinacia oleracea |
1.14.99.22 | 4°C to -20°C storage for prolonged periods not possible due to unstability of the enzyme, appreciable loss of activity | Spinacia oleracea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.22 | alpha-ecdysone + NADPH + O2 | - |
Spinacia oleracea | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
1.14.99.22 | ecdysone + NADPH + O2 | - |
Spinacia oleracea | 20-hydroxyecdysone + NADP+ + H2O | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.99.22 | cytochrome P450 | essential | Spinacia oleracea | |
1.14.99.22 | NADPH | ultimate hydrogen donor, cannot be substituted by NADH or ascorbate | Spinacia oleracea |