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Literature summary extracted from

  • Johnson, P.; Rees, H.H
    The mechanism of C-20 hydroxylation of alpha-ecdysone in the desert locust, Schistocerca gregaria (1977), Biochem. J., 168, 513-520.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.99.22 CO partially reversible by irradiation with monochromatic light Schistocerca gregaria
1.14.99.22 KCN 25% inhibition Schistocerca gregaria
1.14.99.22 Metyrapone 2-methyl-1,2-di-3'-pyridylpropan-1-one, up to 80% inhibition Schistocerca gregaria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.99.22 0.00125
-
alpha-ecdysone
-
Schistocerca gregaria

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.99.22 mitochondrion membrane bound Schistocerca gregaria 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.22 ecdysone + NADPH + O2 Schistocerca gregaria serves as precursor of ecdysterone, the active moulting hormone in larval insects 20-hydroxyecdysone + NADP+ + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.22 Schistocerca gregaria
-
desert locust
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.99.22 ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein Schistocerca gregaria

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.99.22 fat body larval Schistocerca gregaria
-
1.14.99.22 malpighian tubule
-
Schistocerca gregaria
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.99.22 additional information
-
-
Schistocerca gregaria
1.14.99.22 0.00000562
-
malphigian tubules, 10000 x g pellet Schistocerca gregaria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.22 alpha-ecdysone + NADPH + O2
-
Schistocerca gregaria 20-hydroxyecdysone + NADP+ + H2O
-
?
1.14.99.22 ecdysone + NADPH + O2 serves as precursor of ecdysterone, the active moulting hormone in larval insects Schistocerca gregaria 20-hydroxyecdysone + NADP+ + H2O
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.99.22 35
-
-
Schistocerca gregaria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.99.22 6.5
-
-
Schistocerca gregaria

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.22 cytochrome P450 essential Schistocerca gregaria
1.14.99.22 NADPH
-
Schistocerca gregaria