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Literature summary extracted from

  • Lepesheva, G.I.; Azeva, T.N.; Strushkevich, N.V.; Gilep, A.A.; Usanov, S.A.
    Site-directed mutagenesis of cytochrome P450scc (CYP11A1). Effect of lysine residue substitution on its structural and functional properties (2000), Biochemistry (Moscow), 65, 1409-1418.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.6 mutants and wild-type expressed in Escherichia coli Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.6 K103Q decrease of stability Bos taurus
1.14.15.6 K104Q dramatic decrease in expression level Bos taurus
1.14.15.6 K109Q dramatic changes in folding and heme insertion Bos taurus
1.14.15.6 K110Q folding and heme insertion not affected Bos taurus
1.14.15.6 K145Q
-
 Bos taurus
1.14.15.6 K148Q expression level is not affected, extreme instable and rapid denaturation Bos taurus
1.14.15.6 K394Q functional parameters decreased because substitution is close to 405 position, but to much lower extend Bos taurus
1.14.15.6 K403Q functional parameters decreased because substitution is close to 405 position, but to much lower extend, shows importance of this residue for electrostatic interaction with negatively charged residues of ferredoxin Bos taurus
1.14.15.6 K405Q dramatic loss of activity, shows importance of this residue for electrostatic interaction with negatively charged residues of ferredoxin Bos taurus
1.14.15.6 additional information site-directed mutagenesis, no evident changes in functional properties for KQ mutants, 103, 110, 145, 394 and 403 with activities between 69 and 86% of wild-type Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.6 mitochondrial inner membrane inner membrane Bos taurus 5743
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.6 cholesterol + 6 reduced adrenodoxin + 3 O2 Bos taurus
-
 pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O precursor of all steroid hormones ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.6 precipitation with polyethylene glycol, affinity chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.6 adrenal cortex
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.6 cholesterol + 6 reduced adrenodoxin + 3 O2
-
 Bos taurus pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O precursor of all steroid hormones ?
1.14.15.6 cholesterol + reduced adrenodoxin + O2
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.6 adrenodoxin
-
 Bos taurus
1.14.15.6 heme
-
 Bos taurus
1.14.15.6 NADPH NADPH-linked adrenodoxin reductase Bos taurus