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Literature summary extracted from

  • Warne, P.A.; Greenfield, N.J.; Lieberman, S.
    Modulation of the kinetics of cholesterol side-chain cleavage by an activator and by an inhibitor isolated from the cytosol of the cortex of bovine adrenals (1983), Proc. Natl. Acad. Sci. USA, 80, 1877-1881.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.6 additional information activator of cholesterol side-chain cleavage isolated from the cytosol of bovine adrenal cortex Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.6 cholesterol inhibition above 0.003 mM, mitochondrial Bos taurus
1.14.15.6 Cholesterol sulfate inhibition above 0.005 mM, mitochondrial Bos taurus
1.14.15.6 additional information no substrate inhibition with cytosolic enzyme, inhibitor of cholesterol-side-chain cleavage isolated from the cytosol of bovine adrenal cortex Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.6 0.0003
-
 Cholesterol sulfate mitochondrial Bos taurus
1.14.15.6 0.0005
-
 cholesterol mitochondrial Bos taurus
1.14.15.6 0.0165
-
 cholesterol cytosolic Bos taurus
1.14.15.6 0.0232
-
 Cholesterol sulfate cytosolic Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.6 cytosol
-
 Bos taurus 5829
-
1.14.15.6 mitochondrial inner membrane inner membrane Bos taurus 5743
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.6 cholesterol + 6 reduced adrenodoxin + 3 O2 Bos taurus first step in biosynthesis of all steroid hormones pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O isocaproic aldehyde additional product ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.6 column chromatography, gel filtration Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.6 adrenal cortex
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.6 cholesterol + 6 reduced adrenodoxin + 3 O2 first step in biosynthesis of all steroid hormones Bos taurus pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O isocaproic aldehyde additional product ?
1.14.15.6 cholesterol + reduced adrenodoxin + O2
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?
1.14.15.6 cholesterol sulfate + reduced adrenodoxin + O2
-
 Bos taurus pregnenolone sulfate + 17-hydroxy-pregnenolone + dehydroisoandrosterone sulfate + oxidized adrenodoxin + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.6 adrenodoxin
-
 Bos taurus
1.14.15.6 NADPH NADPH-linked adrenodoxin reductase Bos taurus