Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sheets, J.J.; Vickery, L.E.
    Active site-directed inhibitors of cytochrome P-450scc. Structural and mechanistic implications of a side chain-substituted series of amino-steroids (1983), J. Biol. Chem., 258, 11446-11452.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.6 (20R,S)-20-amino-5-pregnen-3beta-ol 20-amine derivative, amine is attached closer to the D-ring than in the 22-amine, very weak inhibitor, 0.1 mM causes less than 20% inhibition Bos taurus
1.14.15.6 17beta-amino-5-androsten-3beta-ol 17-amine derivative, amine is attached closer to the D-ring than in the 22-amine, very weak inhibitor, 0.1 mM causes less than 20% inhibition Bos taurus
1.14.15.6 22-Amino-23,24-bisnor-5-cholen-3beta-ol 22-amine derivative, same steroid ring structure as cholesterol, competitive inhibitor with respect to cholesterol, 50% reversible inhibition at 0.0001 mM, reversible cooperative binding Bos taurus
1.14.15.6 22-amino-23,24-bisnor-5alpha-cholen-3beta-ol 50% inhibition at 0.003 mM Bos taurus
1.14.15.6 23-Amino-24-nor-5-cholen-3beta-ol 23-amine derivative, same steroid ring structure as cholesterol, competitive inhibitor with respect to cholesterol, 50% inhibition at 0.0001 mM, reversible cooperative binding Bos taurus
1.14.15.6 24-Amino-5-cholen-3beta-ol 24-amine derivative, amine attached in greater distance from steroid ring, same steroid ring structure as cholesterol, causes a progressive decrease in inhibitory potency, 50% inhibition at 0.0023 mM, reversible noncooperative binding Bos taurus
1.14.15.6 25-Amino-26,27-bisnor-5-cholesten-3beta-ol 25-amine derivative, amine attached in greater distance from steroid ring, causes a progressive decrease in inhibitory potency, 50% inhibition at more than 0.1 mM Bos taurus
1.14.15.6 additional information cholesterol analogues have shortened side chain and primary amine group, tested in presence of 0.07 mM cholesterol; steroid ring is suggested to bind to the substrate site on the enzyme and the amine is coordinated to the heme iron Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.6 mitochondrial inner membrane
-
Bos taurus 5743
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.6
-
Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.6 adrenal cortex
-
Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.6 cholesterol + reduced adrenodoxin + O2
-
Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.6 37
-
-
Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.6 7.2
-
assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.6 adrenodoxin
-
Bos taurus
1.14.15.6 NADPH
-
Bos taurus