EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.6 | (20R,S)-20-amino-5-pregnen-3beta-ol | 20-amine derivative, amine is attached closer to the D-ring than in the 22-amine, very weak inhibitor, 0.1 mM causes less than 20% inhibition | Bos taurus | |
1.14.15.6 | 17beta-amino-5-androsten-3beta-ol | 17-amine derivative, amine is attached closer to the D-ring than in the 22-amine, very weak inhibitor, 0.1 mM causes less than 20% inhibition | Bos taurus | |
1.14.15.6 | 22-Amino-23,24-bisnor-5-cholen-3beta-ol | 22-amine derivative, same steroid ring structure as cholesterol, competitive inhibitor with respect to cholesterol, 50% reversible inhibition at 0.0001 mM, reversible cooperative binding | Bos taurus | |
1.14.15.6 | 22-amino-23,24-bisnor-5alpha-cholen-3beta-ol | 50% inhibition at 0.003 mM | Bos taurus | |
1.14.15.6 | 23-Amino-24-nor-5-cholen-3beta-ol | 23-amine derivative, same steroid ring structure as cholesterol, competitive inhibitor with respect to cholesterol, 50% inhibition at 0.0001 mM, reversible cooperative binding | Bos taurus | |
1.14.15.6 | 24-Amino-5-cholen-3beta-ol | 24-amine derivative, amine attached in greater distance from steroid ring, same steroid ring structure as cholesterol, causes a progressive decrease in inhibitory potency, 50% inhibition at 0.0023 mM, reversible noncooperative binding | Bos taurus | |
1.14.15.6 | 25-Amino-26,27-bisnor-5-cholesten-3beta-ol | 25-amine derivative, amine attached in greater distance from steroid ring, causes a progressive decrease in inhibitory potency, 50% inhibition at more than 0.1 mM | Bos taurus | |
1.14.15.6 | additional information | cholesterol analogues have shortened side chain and primary amine group, tested in presence of 0.07 mM cholesterol; steroid ring is suggested to bind to the substrate site on the enzyme and the amine is coordinated to the heme iron | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.15.6 | mitochondrial inner membrane | - |
Bos taurus | 5743 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.6 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.15.6 | - |
Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.15.6 | adrenal cortex | - |
Bos taurus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.6 | cholesterol + reduced adrenodoxin + O2 | - |
Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.15.6 | 37 | - |
- |
Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.15.6 | 7.2 | - |
assay at | Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.6 | adrenodoxin | - |
Bos taurus | |
1.14.15.6 | NADPH | - |
Bos taurus |