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Literature summary extracted from

  • Dhariwal, M.S.; Jefcoate, C.R.
    Cholesterol metabolism by purified cytochrome P-450scc is highly stimulated by octyl glucoside and stearic acid exclusively in large unilamellar phospholipid vesicles (1989), Biochemistry, 28, 8397-8402.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.6 cardiolipin affinity of P-450scc for cholesterol is increased Bos taurus
1.14.15.6 fatty acid C18, natural detergents in DMPC vesicles, stimulation similar to octyl glucoside Bos taurus
1.14.15.6 octyl glucoside high concentrations of this detergent cause 50% stimulation of cholesterol side-chain-cleavage in large unilamellar vesicles at low cholesterol concentration, 0.01 mM increase the proportion of P-450 bound by cholesterol Bos taurus
1.14.15.6 Phospholipid P-450scc reconstituted in vesicles, cholate-solubilized for small unilamellar dioleoylphosphatidylcholine vesicles, non-ionic detergent n-octyl glucoside used for large ones Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.6 0.002
-
 cholesterol low concentration, both vesicle types Bos taurus
1.14.15.6 0.3
-
 cholesterol large unilamellar vesicles, second type of reaction Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.6 mitochondrial inner membrane matrix side Bos taurus 5743
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.6 cholesterol + 6 reduced adrenodoxin + 3 O2 Bos taurus first step in biosynthesis of all steroid hormones pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.6 ammonium sulfate precipitation, affinity chromatography Bos taurus
1.14.15.6 DEAE-cellulose, adrenodoxin-Sepharose Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.6 adrenal cortex
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.6 cholesterol + 6 reduced adrenodoxin + 3 O2 first step in biosynthesis of all steroid hormones Bos taurus pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
 ?
1.14.15.6 cholesterol + reduced adrenodoxin + O2
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?
1.14.15.6 additional information transfer of cholesterol to cytochrome P-450scc is rate-limiting Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.6 37
-
 assay at Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.15.6 additional information
-
 additional information for small unilamellar vesicles 5fold lower Bos taurus
1.14.15.6 0.417 0.5 cholesterol by large unilamellar vesicles Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.6 7.2
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.6 adrenodoxin required Bos taurus
1.14.15.6 NADPH
-
 Bos taurus