BRENDA - Enzyme Database

Purification and characterization of adrenal cortex mitochondrial cytochrome P-450 specific for cholesterol side chain cleavage activity

Wang, H.P.; Kimura, T.; J. Biol. Chem. 251, 6068-6074 (1976)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.15.6
phosphatidyl choline
-
Bos taurus
1.14.15.6
phosphatidyl ethanolamine
-
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.15.6
mitochondrial inner membrane
-
Bos taurus
5743
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.15.6
46000
-
x * 60000, SDS-PAGE, molecular weights of bovine P-450 reported: 1 * 46000 and x * 53000
Bos taurus
1.14.15.6
53000
-
x * 60000, SDS-PAGE, molecular weights of bovine P-450 reported: 1 * 46000 and x * 53000
Bos taurus
1.14.15.6
60000
-
x * 60000, SDS-PAGE, molecular weights of bovine P-450 reported: 1 * 46000 and x * 53000
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.6
cholesterol + 6 reduced adrenodoxin + 3 O2
Bos taurus
first step in biosynthesis of all steroid hormones
pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
Bos taurus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.6
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.15.6
cholate extraction, pyrophosphate treatment, affinity chromatography
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.15.6
adrenal cortex
-
Bos taurus
-
Storage Stability
EC Number
Storage Stability
Organism
1.14.15.6
-20°C, 10 mM sodium phosphate buffer, pH 7.4, 0.1 mM EDTA, 20% glycerol, stable for months
Bos taurus
1.14.15.6
-20°C, mitochondrial precipitate, 10 mM sodium phosphate buffer, pH 7.4, 0.1 mM EDTA
Bos taurus
1.14.15.6
-20°C, purified, 10 mM sodium phosphate buffer, pH 7.4, 0.1 mM EDTA, 20% glycerol, for months without loss of P-450 activity
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.6
cholesterol + 6 reduced adrenodoxin + 3 O2
first step in biosynthesis of all steroid hormones
285415
Bos taurus
pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
285415
Bos taurus
?
1.14.15.6
cholesterol + reduced adrenodoxin + O2
-
285415
Bos taurus
pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
285415
Bos taurus
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.15.6
?
x * 60000, SDS-PAGE, molecular weights of bovine P-450 reported: 1 * 46000 and x * 53000
Bos taurus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.15.6
37
-
assay at
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.15.6
7.4
-
assay at
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.6
adrenodoxin
-
Bos taurus
1.14.15.6
heme
low heme content
Bos taurus
1.14.15.6
NADPH
-
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.6
adrenodoxin
-
Bos taurus
1.14.15.6
heme
low heme content
Bos taurus
1.14.15.6
NADPH
-
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.15.6
phosphatidyl choline
-
Bos taurus
1.14.15.6
phosphatidyl ethanolamine
-
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.15.6
mitochondrial inner membrane
-
Bos taurus
5743
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.15.6
46000
-
x * 60000, SDS-PAGE, molecular weights of bovine P-450 reported: 1 * 46000 and x * 53000
Bos taurus
1.14.15.6
53000
-
x * 60000, SDS-PAGE, molecular weights of bovine P-450 reported: 1 * 46000 and x * 53000
Bos taurus
1.14.15.6
60000
-
x * 60000, SDS-PAGE, molecular weights of bovine P-450 reported: 1 * 46000 and x * 53000
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.6
cholesterol + 6 reduced adrenodoxin + 3 O2
Bos taurus
first step in biosynthesis of all steroid hormones
pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
Bos taurus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.6
cholate extraction, pyrophosphate treatment, affinity chromatography
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.15.6
adrenal cortex
-
Bos taurus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.14.15.6
-20°C, 10 mM sodium phosphate buffer, pH 7.4, 0.1 mM EDTA, 20% glycerol, stable for months
Bos taurus
1.14.15.6
-20°C, mitochondrial precipitate, 10 mM sodium phosphate buffer, pH 7.4, 0.1 mM EDTA
Bos taurus
1.14.15.6
-20°C, purified, 10 mM sodium phosphate buffer, pH 7.4, 0.1 mM EDTA, 20% glycerol, for months without loss of P-450 activity
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.6
cholesterol + 6 reduced adrenodoxin + 3 O2
first step in biosynthesis of all steroid hormones
285415
Bos taurus
pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
285415
Bos taurus
?
1.14.15.6
cholesterol + reduced adrenodoxin + O2
-
285415
Bos taurus
pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
285415
Bos taurus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.15.6
?
x * 60000, SDS-PAGE, molecular weights of bovine P-450 reported: 1 * 46000 and x * 53000
Bos taurus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.15.6
37
-
assay at
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.15.6
7.4
-
assay at
Bos taurus