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Literature summary extracted from
- Photoaffinity labeling of cytochrome P-45011beta with methyltrienolone as a probe for the substrate binding region (1993), Biochim. Biophys. Acta, 1161, 257-264.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.4 | methyltrienolone | synthetic androgen, used as photoaffinity ligand and substrate analog, covalent binding, 0.1 mM inhibits cortisol synthesis, during photolabeling radioactivity incorporation via radioactive methyltrienolone is blocked by 11-deoxycorticosterone, so it binds to the conserved substrate binding region Trp428-Leu429-Asp430-Arg431 between beta3-sheet and the L-helix analysed by trypsin digest | Bos taurus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.15.4 | 0.0008 | - |
11-deoxycorticosterone | presence of methyltrienolone | Bos taurus | |
| 1.14.15.4 | 0.002 | - |
11-deoxycorticosterone | absence of methyltrienolone | Bos taurus | |
| 1.14.15.4 | 0.06 | 0.09 | corticosterone | presence of methyltrienolone | Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.15.4 | mitochondrion | - |
Bos taurus | 5739 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.4 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.15.4 | RP HPLC for detection of photolabeled peptide after digest with TPCK-treated trypsin of purified cytochrome P-45011beta, which is photolabeled with radioactive methyltrienolone | Bos taurus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.15.4 | a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O | superfamily of heme-containing enzyme, 11-/18-/19-hydroxylations of 11-deoxycorticosterone, 11-deoxycortisol and androstenedione | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.15.4 | adrenal cortex | - |
Bos taurus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.15.4 | -80°C, 20 mM potassium phosphate buffer, pH 7.4, 0.01 mM 11-deoxycorticosterone, 0.1 mM EDTA, 0.1 mM dithiothreitol, 1%, w/v, sodium cholate | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.4 | 11-deoxycortisol + reduced adrenal ferredoxin + O2 | cytochrome P-45011beta-linked monoxygenase | Bos taurus | cortisol + oxidized adrenal ferredoxin + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.15.4 | cytochrome P-45011-beta | - |
Bos taurus |
| 1.14.15.4 | P-450XIB1 | - |
Bos taurus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.4 | 37 | - |
assay at | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.4 | 7.4 | - |
assay at | Bos taurus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.4 | NADPH | adrenodoxin and NADPH-linked adrenodoxin reductase | Bos taurus | |
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Release 2023.1 (January 2023)
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