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Literature summary extracted from
- Mechanism-based inactivation of bovine cytochrome P-45011beta by 18-unsaturated progesterone derivatives (1997), Eur. J. Biochem., 248, 252-260.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.14.15.4 | medicine | inhibitors for P-45011beta, against aldosterone overproduction which leads to oedematous diseases and hypertension | Bos taurus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.15.4 | deoxycorticosterone, protection of enzyme from inactivation by 18-ethynylprogesterone | Bos taurus |
| 1.14.15.4 | phospholipid vesicles, 0.2% soybean lecithin, added to purified P-45011beta for storage and during incubation | Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.4 | 18-ethynylprogesterone | mechanism-based inhibition; progesterone analog, time-dependent pseudo-first-order inactivation, concentration dependent | Bos taurus |  |
| 1.14.15.4 | 18-vinylprogesterone | mechanism-based inhibition; progesterone analog, with NADPH, time and concentration dependent, irreversible, pseudo-first-order process, covalent binding to and destruction of prosthetic heme group, 5fold more effective than its acetylenic analog 18-ethynylprogesterone | Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.15.4 | mitochondrion | - |
Bos taurus | 5739 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.4 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.15.4 | copurification of P-450 11beta-2 and 11beta-3, ammonium sulfate precipitation, octyl-Sepharose, separation of 2 isoforms by hydroxyapatite column connected to an FPLC system | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.15.4 | adrenal cortex | - |
Bos taurus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.15.4 | -80°C, after octyl-Sepharose, deoxycorticosterone-bound, 50 mM potassium phosphate, pH 7.4, 0.5% sodium cholate, 0.2% Tween 20, 0.01 mM 11-deoxycorticosterone, 0.1 mM dithiothreitol, 0.1 mM EDTA | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.4 | 11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 | 3 step 11beta-/18-hydroxylation to aldosterone | Bos taurus | aldosterone + oxidized adrenal ferredoxin + 2 H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.4 | 30 | - |
assay at | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.4 | 7.4 | - |
assay at | Bos taurus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.4 | NADPH | requirement during inactivation | Bos taurus | |
| 1.14.15.4 | NADPH | adrenodoxin and NADPH-linked adrenodoxin reductase | Bos taurus | |
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Release 2023.1 (January 2023)
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