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Literature summary extracted from

  • Yanigabashi, K.; Kobayashi, Y.; Hall, P.F.
    Ascorbate as a source of reducing equivalents for the synthesis of aldosterone (1990), Biochem. Biophys. Res. Commun., 170, 1256-1262.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.4 malate plus NADP+ and in synergism with ascorbate plus NADH increase of aldosterone synthetase activity in zona glomerulosa, no stimulation of 11beta-/18-hydroxylation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.4 ascorbate without NADH, inhibition of aldosterone synthetase Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.4 mitochondrion from zona fasciculata Bos taurus 5739
-
1.14.15.4 mitochondrion from zona glomerulosa and fasciculata Bos taurus 5739
-
1.14.15.4 mitochondrion from zona glomerulosa Bos taurus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.4 Bos taurus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.4 a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O for synthesis of aldosterone 3 molecules P450 must be reduced or one molecule must be reduced 3 times Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.4 adrenal cortex
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.4 11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 3 steps in glomerulosa catalysed by one enzyme, 11beta-hydroxylation to corticosterone, 18-hydroxylation to 18-hydroxycorticosterone and aldehyde synthesis to aldosterone Bos taurus aldosterone + oxidized adrenal ferredoxin + 2 H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.4 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.4 7.4
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.4 ascorbate plus NADH very low conversion and no aldosterone production, in synergism with NADH, malate and NADP+ increase of aldosterone synthetase activity in zona glomerulosa, not zona fasciculate Bos taurus
1.14.15.4 NADH
-
 Bos taurus
1.14.15.4 NADP+ plus malate supports all 4 conversions, increase of aldosterone synthetase activity Bos taurus
1.14.15.4 NADPH
-
 Bos taurus