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Literature summary extracted from

  • Sato, H.; Ashida, N.; Suhara, K.; Itagaki, E.; Takemori, S.; Katagiri, M.
    Properties of an adrenal cytochrome P-450 (P-45011beta) for the hydroxylations of corticosteroids (1978), Arch. Biochem. Biophys., 190, 307-314.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.4 CO affects 11beta-/19-hydroxylase reaction Bos taurus
1.14.15.4 Metyrapone competitive with substrate, affects strongly 11beta-/18-hydroxylation and 11beta-/19-hydroxylation Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.4 0.006
-
 deoxycorticosterone identical for both enzyme activities Bos taurus
1.14.15.4 0.013
-
 4-androstene-3,17-dione identical for both enzyme activities Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.4 mitochondrion
-
 Bos taurus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.4 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.4 copurification of 11beta-/18-hydroxylase with 11beta-/19-hydroxylase, cholate extraction Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.4 a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O P-45011beta responsible for two hydroxylase reactions Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.4 adrenal cortex
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.4 additional information
-
 activities of sonicated mitochondria and cholate extract of hydroxylation activities with deoxycotricosterone and 4-androstene-3,17-dione as substrates Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.14.15.4 -80°C, 59 mM potassium phosphate buffer, pH 7.4, 0.1 mM EDTA, 0.1 mM dithiothreitol, 0.01 mM deoxycorticosterone, 0.3% cholate, 0.3% Tween 20 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.4 11-deoxycorticosterone + reduced adrenal ferredoxin + O2 containing adrenal ferredoxin, adrenal ferredoxin reductase and P-45011beta, highly active hydroxylation, 11beta-/18-hydroxylase activity Bos taurus corticosterone + oxidized adrenal ferredoxin + H2O
-
 ?
1.14.15.4 4-androstene-3,17-dione + reduced adrenal ferredoxin + O2 i.e. testosterone, 11beta-/19-hydroxylase activity, good sustrate Bos taurus 11beta-hydroxy-4-androstene-3,17-dione + oxidized adrenal ferredoxin + H2O
-
 ?
1.14.15.4 4-androstene-3,17-dione + reduced adrenal ferredoxin + O2 i.e. testosterone, 11beta-/19-hydroxylase activity, good sustrate Bos taurus 19-hydroxy-4-androstene-3,17-dione + oxidized adrenal ferredoxin + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.4 37
-
 assay at Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.15.4 0.3
-
 11-deoxycorticosterone 18-hydroxylation Bos taurus
1.14.15.4 1.83
-
 11-deoxycorticosterone 11beta-hydroxylation Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.4 7.4
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.4 NADPH
-
 Bos taurus