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Literature summary extracted from
- Structural determination of the substrate specificities and regioselectivities of the rat and human fatty acid omega-hydroxylases (2000), Arch. Biochem. Biophys., 373, 63-71.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.80 | expression in Escherichia coli | Homo sapiens |
| 1.14.14.80 | expression in Escherichia coli | Rattus norvegicus |
| 1.14.15.3 | expressed in Escherichia coli, genes CYP4A1, CYP4A2, CYP4A3 and CYP4A8 | Rattus norvegicus |
| 1.14.15.3 | expressed in Escherichia coli, human gene CYP4A11 | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.14.80 | additional information | construction of two complementary chimera between isoforms CYP4A2-CYP4A3 fused at residue 119 (CYP4A2) and 122 (CYP4A3). The chimera in which the first 119 amino acids are from CYP4A2 indicates that the first 120 amino acids control the substrate specificity. The chimera in which the first 122 amino acids are from CYP4A3 is inactive due to a defect in electron transfer to the heme group | Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.80 | 0.003 | - |
arachidonic acid | pH 7.4, 37°C | Rattus norvegicus |  |
| 1.14.14.80 | 0.004 | - |
myristic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.01 | - |
arachidonic acid | pH 7.4, 37°C | Homo sapiens | |
| 1.14.14.80 | 0.013 | - |
myristic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.018 | - |
oleic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.019 | - |
arachidonic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.022 | - |
lauric acid | pH 7.4, 37°C | Homo sapiens | |
| 1.14.14.80 | 0.03 | - |
lauric acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.046 | - |
myristic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.064 | - |
oleic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.07 | - |
myristic acid | pH 7.4, 37°C | Homo sapiens | |
| 1.14.14.80 | 0.08 | - |
lauric acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.136 | - |
oleic acid | pH 7.4, 37°C | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.3 | arachidonic acid + NADPH + H+ + O2 | Homo sapiens | - |
20-hydroxyeicosatetraenoic acid + NADP+ + H2O | - |
? | |
| 1.14.15.3 | additional information | Homo sapiens | 20-hydroxyeicosatetraenoic acid modulates renal transport activities | ? | - |
? | |
| 1.14.15.3 | n-alkane + NAD(P)H + H+ + O2 | Homo sapiens | - |
n-alkanol + NAD(P)+ + H2O | - |
? | |
| 1.14.15.3 | n-alkane + NAD(P)H + H+ + O2 | Rattus norvegicus | - |
n-alkanol + NAD(P)+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.80 | Homo sapiens | Q02928 | - |
- |
| 1.14.14.80 | Rattus norvegicus | P08516 | - |
- |
| 1.14.14.80 | Rattus norvegicus | P20816 | - |
- |
| 1.14.14.80 | Rattus norvegicus | P20817 | - |
- |
| 1.14.14.80 | Rattus norvegicus | P24464 | - |
- |
| 1.14.15.3 | Homo sapiens | - |
- |
- |
| 1.14.15.3 | Rattus norvegicus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.80 | kidney | - |
Homo sapiens | - |
| 1.14.14.80 | kidney | - |
Rattus norvegicus | - |
| 1.14.15.3 | liver | - |
Rattus norvegicus | - |
| 1.14.15.3 | renal cortex | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.80 | arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 20-hydroxyarachidonic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rattus norvegicus | 20-hydroxyarachidonic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2 | regioselectivity of omega:omega-1 hydroxylation is 2.4:1 | Rattus norvegicus | 20-hydroxyarachidonic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2 | regioselectivity of omega:omega-1 hydroxylation is 2:1 | Rattus norvegicus | 20-hydroxyarachidonic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2 | regioselectivity of omega:omega-1 hydroxylation is 6:1 | Rattus norvegicus | 20-hydroxyarachidonic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | lauric acid + [reduced NADPH-hemoprotein reductase] + O2 | regioselectivity of omega:omega-1 hydroxylation is 2.5:1 | Homo sapiens | 12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | lauric acid + [reduced NADPH-hemoprotein reductase] + O2 | regioselectivity of omega:omega-1 hydroxylation is 2.5:1 | Rattus norvegicus | 12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | lauric acid + [reduced NADPH-hemoprotein reductase] + O2 | regioselectivity of omega:omega-1 hydroxylation is 3:1 | Rattus norvegicus | 12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | lauric acid + [reduced NADPH-hemoprotein reductase] + O2 | regioselectivity of omega:omega-1 hydroxylation is 40:1 | Rattus norvegicus | 12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | lauric acid + [reduced NADPH-hemoprotein reductase] + O2 | regioselectivity of omega:omega-1 hydroxylation is 6:1 | Rattus norvegicus | 12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | myristic acid + [reduced NADPH-hemoprotein reductase] + O2 | regioselectivity of omega:omega-1 hydroxylation is 1.2:1 | Rattus norvegicus | 14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | myristic acid + [reduced NADPH-hemoprotein reductase] + O2 | regioselectivity of omega:omega-1 hydroxylation is 1.6:1 | Homo sapiens | 14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | myristic acid + [reduced NADPH-hemoprotein reductase] + O2 | regioselectivity of omega:omega-1 hydroxylation is 1.6:1 | Rattus norvegicus | 14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | myristic acid + [reduced NADPH-hemoprotein reductase] + O2 | regioselectivity of omega:omega-1 hydroxylation is 3:1 | Rattus norvegicus | 14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | oleic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 18-hydroxyoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | oleic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rattus norvegicus | 18-hydroxyoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rattus norvegicus | 16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 | regioselectivity of omega:omega-1 hydroxylation is 1.6:1 | Homo sapiens | 16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 | regioselectivity of omega:omega-1 hydroxylation is 1.6:1 | Rattus norvegicus | 16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.80 | palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 | regioselectivity of omega:omega-1 hydroxylation is 1:1 | Rattus norvegicus | 16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.15.3 | arachidonic acid + NAD(P)H + H+ + O2 | - |
Homo sapiens | 20-hydroxyicosa-5,8,11,14-tetraenoic acid + NAD(P)+ + H2O | - |
? | |
| 1.14.15.3 | arachidonic acid + NAD(P)H + H+ + O2 | - |
Rattus norvegicus | 20-hydroxyicosa-5,8,11,14-tetraenoic acid + NAD(P)+ + H2O | - |
? | |
| 1.14.15.3 | arachidonic acid + NADPH + H+ + O2 | - |
Homo sapiens | 20-hydroxyeicosatetraenoic acid + NADP+ + H2O | - |
? | |
| 1.14.15.3 | fatty acid + NAD(P)H + H+ + O2 | chain length C12-C16, arachidonic acid and oleic acid | Homo sapiens | omega-hydroxy fatty acid + NAD(P)+ + H2O | - |
? | |
| 1.14.15.3 | fatty acid + NAD(P)H + H+ + O2 | chain length C12-C16, arachidonic acid and oleic acid | Rattus norvegicus | omega-hydroxy fatty acid + NAD(P)+ + H2O | - |
? | |
| 1.14.15.3 | lauric acid + NAD(P)H + H+ + O2 | - |
Homo sapiens | 12-hydroxydodecanoic acid + NAD(P)+ + H2O | - |
? | |
| 1.14.15.3 | lauric acid + NAD(P)H + H+ + O2 | - |
Rattus norvegicus | 12-hydroxydodecanoic acid + NAD(P)+ + H2O | - |
? | |
| 1.14.15.3 | additional information | 20-hydroxyeicosatetraenoic acid modulates renal transport activities | Homo sapiens | ? | - |
? | |
| 1.14.15.3 | n-alkane + NAD(P)H + H+ + O2 | - |
Homo sapiens | n-alkanol + NAD(P)+ + H2O | - |
? | |
| 1.14.15.3 | n-alkane + NAD(P)H + H+ + O2 | - |
Rattus norvegicus | n-alkanol + NAD(P)+ + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.80 | CYP4A1 | - |
Rattus norvegicus |
| 1.14.14.80 | CYP4A11 | - |
Homo sapiens |
| 1.14.14.80 | CYP4A2 | - |
Rattus norvegicus |
| 1.14.14.80 | CYP4A3 | - |
Rattus norvegicus |
| 1.14.14.80 | CYP4A8 | - |
Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.80 | 0.01 | - |
arachidonic acid | pH 7.4, 37°C | Homo sapiens | |
| 1.14.14.80 | 0.01 | - |
oleic acid | pH 7.4, 37°C | Homo sapiens | |
| 1.14.14.80 | 0.01 | - |
oleic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.02 | - |
arachidonic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.02 | - |
oleic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.03 | - |
arachidonic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.03 | - |
palmitic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.1 | - |
arachidonic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.1 | - |
palmitic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.1 | - |
myristic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.17 | - |
palmitic acid | pH 7.4, 37°C | Homo sapiens | |
| 1.14.14.80 | 0.58 | - |
lauric acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.67 | - |
myristic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 0.7 | - |
lauric acid | pH 7.4, 37°C | Homo sapiens | |
| 1.14.14.80 | 0.83 | - |
myristic acid | pH 7.4, 37°C | Homo sapiens | |
| 1.14.14.80 | 1 | - |
palmitic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 1.17 | - |
myristic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 1.22 | - |
lauric acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 3.8 | - |
myristic acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 3.8 | - |
lauric acid | pH 7.4, 37°C | Rattus norvegicus | |
| 1.14.14.80 | 10.8 | - |
lauric acid | pH 7.4, 37°C | Rattus norvegicus | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.3 | NADPH | - |
Homo sapiens | |
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