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Literature summary extracted from

  • Dierks, E.A.; Davis, S.C.; Ortiz de Montellano, P.R.
    Glu-325 and Asp-328 are determinants of the cyp4A1 hydroxylation regiospecificity and resistance to inactivation by 1-aminobenzotriazole (1998), Biochemistry, 37, 1839-1847.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.3 expressed in Escherichia coli XL-1 blue, human gene CYP4A1 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.3 D323E gene CYP4A1 Homo sapiens
1.14.15.3 E320A gene CYP4A1 Homo sapiens
1.14.15.3 E320A/D323E gene CYP4A1 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.3 0.029
-
 lauric acid D323E mutant Homo sapiens
1.14.15.3 0.031
-
 lauric acid E320A/D323E mutant Homo sapiens
1.14.15.3 0.036
-
 lauric acid E320A mutant Homo sapiens
1.14.15.3 0.16
-
 lauric acid
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.3 arachidonic acid + NADPH + H+ + O2 Homo sapiens
-
 20-hydroxyeicosatetraenoic acid + NADP+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Homo sapiens
-
 n-alkanol + NAD(P)+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.3 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.3 arachidonic acid + NADPH + H+ + O2
-
 Homo sapiens 20-hydroxyeicosatetraenoic acid + NADP+ + H2O
-
 ?
1.14.15.3 lauric acid + NAD(P)H + H+ + O2
-
 Homo sapiens 12-hydroxydodecanoic acid + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
 Homo sapiens n-alkanol + NAD(P)+ + H2O
-
 ?