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Literature summary extracted from

  • Scheller, U.; Zimmer, T.; Kaergel, E.; Schunck, W.H.
    Characterization of the n-alkane and fatty acid hydroxylating cytochrome P450 forms 52A3 and 52A4 (1996), Arch. Biochem. Biophys., 328, 245-254.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.3 Emulgen 911 needs detergent 0.3% Candida maltosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.3 expressed in Saccharomyces cerevisiae strain GRF18 Candida maltosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.3 microsome integral membrane protein Candida maltosa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.3 Fe
-
Candida maltosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.3 55000
-
gene expression CYP52A3, SDS-PAGE Candida maltosa
1.14.15.3 56500
-
gene expression CYP52A4, SDS-PAGE Candida maltosa
1.14.15.3 59800
-
gene expression CYP52A3, amino acid composition Candida maltosa
1.14.15.3 61800
-
gene expression CYP52A4, amino acid composition Candida maltosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Candida maltosa
-
n-alkanol + NAD(P)+ + H2O
-
?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Candida maltosa EH15
-
n-alkanol + NAD(P)+ + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.3 Candida maltosa
-
-
-
1.14.15.3 Candida maltosa EH15
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.3
-
Candida maltosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.3 fatty acid + NAD(P)H + H+ + O2 protein from CYP52A3 has a higher affinity for fatty acids than for alkanes Candida maltosa omega-hydroxy fatty acid + NAD(P)+ + H2O
-
?
1.14.15.3 fatty acid + NAD(P)H + H+ + O2 protein from CYP52A3 has a higher affinity for fatty acids than for alkanes Candida maltosa EH15 omega-hydroxy fatty acid + NAD(P)+ + H2O
-
?
1.14.15.3 lauric acid + NAD(P)H + H+ + O2
-
Candida maltosa 12-hydroxydodecanoic acid + NAD(P)+ + H2O
-
?
1.14.15.3 lauric acid + NAD(P)H + H+ + O2
-
Candida maltosa EH15 12-hydroxydodecanoic acid + NAD(P)+ + H2O
-
?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
Candida maltosa n-alkanol + NAD(P)+ + H2O
-
?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 protein from CYP52A3 has a higher affinity for alkanes than for fatty acids Candida maltosa n-alkanol + NAD(P)+ + H2O
-
?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
Candida maltosa EH15 n-alkanol + NAD(P)+ + H2O
-
?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 protein from CYP52A3 has a higher affinity for alkanes than for fatty acids Candida maltosa EH15 n-alkanol + NAD(P)+ + H2O
-
?
1.14.15.3 palmitic acid + NAD(P)H + H+ + O2
-
Candida maltosa 16-hydroxyhexadecanoic acid + NAD(P)+ + H2O
-
?
1.14.15.3 palmitic acid + NAD(P)H + H+ + O2
-
Candida maltosa EH15 16-hydroxyhexadecanoic acid + NAD(P)+ + H2O
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.3 7.4
-
assay conditions Candida maltosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.3 NADPH
-
Candida maltosa