EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.3 | KCN | reversible inhibitor | Pseudomonas oleovorans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.15.3 | cell membrane | membrane-bound enzyme, needs 20 molecules phospholipid per polypeptide chain | Pseudomonas oleovorans | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.3 | Fe | 1 atom Fe per 42000 g/mol peptide | Pseudomonas oleovorans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.3 | n-alkane + NAD(P)H + H+ + O2 | Pseudomonas oleovorans | - |
n-alkanol + NAD(P)+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.3 | Pseudomonas oleovorans | - |
- |
- |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.14.15.3 | ferrous ions restore activity after treatment with EDTA and dithionite | Pseudomonas oleovorans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.15.3 | 2 | - |
- |
Pseudomonas oleovorans |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.15.3 | -20°C, 1 month, under 50% activity retained | Pseudomonas oleovorans |
1.14.15.3 | -70°C, 12 days, over 90% activity retained | Pseudomonas oleovorans |
1.14.15.3 | -70°C, 6 months, over 50% activity retained | Pseudomonas oleovorans |
1.14.15.3 | 4°C, 5 days, under 50% activity retained | Pseudomonas oleovorans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.3 | 1,7-octadiene + NADH + H+ + O2 | epoxidation of simple, aliphatic terminal olefins | Pseudomonas oleovorans | 1,2-epoxy-7-octene + NAD+ + H2O | - |
? | |
1.14.15.3 | 2,5-dimethylhexane + NADH + H+ + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
1.14.15.3 | cyclohexane + NADH + H+ + O2 | - |
Pseudomonas oleovorans | cyclohexanol + NAD+ + H2O | - |
? | |
1.14.15.3 | fatty acid + NAD(P)H + H+ + O2 | chain length C6-C14 | Pseudomonas oleovorans | omega-hydroxy fatty acid + NAD(P)+ + H2O | - |
? | |
1.14.15.3 | methylcyclohexane + NADH + H+ + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
1.14.15.3 | n-alkane + NAD(P)H + H+ + O2 | - |
Pseudomonas oleovorans | n-alkanol + NAD(P)+ + H2O | - |
? | |
1.14.15.3 | n-alkane + NAD(P)H + H+ + O2 | rubredoxin is electron carrier | Pseudomonas oleovorans | n-alkanol + NAD(P)+ + H2O | - |
? | |
1.14.15.3 | n-alkane + NAD(P)H + H+ + O2 | chain length C6-C14 | Pseudomonas oleovorans | n-alkanol + NAD(P)+ + H2O | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.15.3 | 30 | - |
assay conditions | Pseudomonas oleovorans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.15.3 | 7.4 | - |
assay conditions | Pseudomonas oleovorans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.3 | NADH | hydroxylation needs also a NADH-dependent bacterial reductase | Pseudomonas oleovorans |