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Literature summary extracted from

  • Ruettinger, R.T.; Griffith, G.R.; Coon, M.J.
    Characterization of the omega-hydroxylase of Pseudomonas oleovorans as a nonheme iron protein (1977), Arch. Biochem. Biophys., 183, 528-537.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.15.3 after 24 h 15 mM EDTA 60% activity and iron retained Pseudomonas oleovorans
1.14.15.3 after 4 h 15 mM EDTA and 10 mM dithionite 10% activity and iron retained Pseudomonas oleovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.3 KCN reversible inhibitor Pseudomonas oleovorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.3 cell membrane membrane-bound enzyme, needs 20 molecules phospholipid per polypeptide chain Pseudomonas oleovorans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.3 Cu 0.03 atoms per 42000 g/mol peptide Pseudomonas oleovorans
1.14.15.3 Fe 1 atom Fe per 42000 g/mol peptide Pseudomonas oleovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.3 40100
-
 x * 40100, amino acid composition Pseudomonas oleovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Pseudomonas oleovorans
-
 n-alkanol + NAD(P)+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.3 Pseudomonas oleovorans
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.15.3 glycoprotein 5% carbohydrate Pseudomonas oleovorans

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.3
-
 Pseudomonas oleovorans

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.14.15.3 ferrous ions restore activity after treatment with EDTA and dithionite Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.3 1.23
-
 hydroxylation of octane Pseudomonas oleovorans
1.14.15.3 1.75
-
 epoxidation of 1,7-octadiene Pseudomonas oleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.3 1,7-octadiene + NADH + H+ + O2 epoxidation of simple, aliphatic terminal olefins Pseudomonas oleovorans 1,2-epoxy-7-octene + NAD+ + H2O
-
 ?
1.14.15.3 fatty acid + NAD(P)H + H+ + O2
-
 Pseudomonas oleovorans omega-hydroxy fatty acid + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
 Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 rubredoxin is electron carrier Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.3 ? x * 40100, amino acid composition Pseudomonas oleovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.3 NADH hydroxylation needs also a NADH-dependent bacterial reductase Pseudomonas oleovorans