BRENDA - Enzyme Database

Enzymatic omega-oxidation. IV. Purification and properties of the omega-hydroxylase of Pseudomonas oleovorans

McKenna, E.J.; Coon, M.J.; J. Biol. Chem. 245, 3882-3889 (1970)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.15.3
0.032
-
laurate
-
Pseudomonas oleovorans
1.14.15.3
0.69
-
Nonanoate
-
Pseudomonas oleovorans
1.14.15.3
5.2
-
Heptanoate
-
Pseudomonas oleovorans
1.14.15.3
22
-
hexanoate
-
Pseudomonas oleovorans
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.15.3
Fe
non-heme iron protein
Pseudomonas oleovorans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.15.3
42000
-
x * 42000, SDS-PAGE
Pseudomonas oleovorans
1.14.15.3
2000000
-
gel filtration
Pseudomonas oleovorans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.3
n-alkane + NAD(P)H + H+ + O2
Pseudomonas oleovorans
-
n-alkanol + NAD(P)+ + H2O
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.3
Pseudomonas oleovorans
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.15.3
-
Pseudomonas oleovorans
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.15.3
596
-
-
Pseudomonas oleovorans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.3
2,5-dimethylhexane + NADH + H+ + O2
-
285363
Pseudomonas oleovorans
?
-
-
-
?
1.14.15.3
cyclohexane + NADH + H+ + O2
-
285363
Pseudomonas oleovorans
cyclohexanol + NAD+ + H2O
-
-
-
?
1.14.15.3
fatty acid + NAD(P)H + H+ + O2
chain length C6-C11, maximal activity with heptanoate
285363
Pseudomonas oleovorans
omega-hydroxy fatty acid + NAD(P)+ + H2O
-
-
-
?
1.14.15.3
lauric acid + NAD(P)H + H+ + O2
-
285363
Pseudomonas oleovorans
12-hydroxydodecanoic acid + NAD(P)+ + H2O
-
-
-
?
1.14.15.3
lecithin + NADH + H+ + O2
-
285363
Pseudomonas oleovorans
?
-
-
-
?
1.14.15.3
methylcyclohexane + NADH + H+ + O2
-
285363
Pseudomonas oleovorans
?
-
-
-
?
1.14.15.3
monoolein + NADH + H+ + O2
-
285363
Pseudomonas oleovorans
18-hydroxyoctadec-9-enoic acid 2,3-dihydroxypropyl ester + NAD+ + H2O
-
-
-
?
1.14.15.3
n-alkane + NAD(P)H + H+ + O2
-
285363
Pseudomonas oleovorans
n-alkanol + NAD(P)+ + H2O
-
-
-
-
1.14.15.3
n-alkane + NAD(P)H + H+ + O2
rubredoxin is electron carrier
285363
Pseudomonas oleovorans
n-alkanol + NAD(P)+ + H2O
-
-
-
?
1.14.15.3
n-alkane + NAD(P)H + H+ + O2
chain length C6-C16, maximal activity with n-octane
285363
Pseudomonas oleovorans
n-alkanol + NAD(P)+ + H2O
-
-
-
?
1.14.15.3
phosphatidylethanolamine + NADH + H+ + O2
-
285363
Pseudomonas oleovorans
?
-
-
-
?
1.14.15.3
phosphatidylserine + NADH + H+ + O2
-
285363
Pseudomonas oleovorans
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.15.3
?
x * 42000, SDS-PAGE
Pseudomonas oleovorans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.15.3
7.5
-
Tris buffer
Pseudomonas oleovorans
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.14.15.3
6.7
7.5
laurate hydroxylation in Tris buffer
Pseudomonas oleovorans
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.3
NADH
hydroxylation needs also a NADH-dependent bacterial reductase
Pseudomonas oleovorans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.3
NADH
hydroxylation needs also a NADH-dependent bacterial reductase
Pseudomonas oleovorans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.15.3
0.032
-
laurate
-
Pseudomonas oleovorans
1.14.15.3
0.69
-
Nonanoate
-
Pseudomonas oleovorans
1.14.15.3
5.2
-
Heptanoate
-
Pseudomonas oleovorans
1.14.15.3
22
-
hexanoate
-
Pseudomonas oleovorans
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.15.3
Fe
non-heme iron protein
Pseudomonas oleovorans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.15.3
42000
-
x * 42000, SDS-PAGE
Pseudomonas oleovorans
1.14.15.3
2000000
-
gel filtration
Pseudomonas oleovorans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.3
n-alkane + NAD(P)H + H+ + O2
Pseudomonas oleovorans
-
n-alkanol + NAD(P)+ + H2O
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.3
-
Pseudomonas oleovorans
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.15.3
596
-
-
Pseudomonas oleovorans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.3
2,5-dimethylhexane + NADH + H+ + O2
-
285363
Pseudomonas oleovorans
?
-
-
-
?
1.14.15.3
cyclohexane + NADH + H+ + O2
-
285363
Pseudomonas oleovorans
cyclohexanol + NAD+ + H2O
-
-
-
?
1.14.15.3
fatty acid + NAD(P)H + H+ + O2
chain length C6-C11, maximal activity with heptanoate
285363
Pseudomonas oleovorans
omega-hydroxy fatty acid + NAD(P)+ + H2O
-
-
-
?
1.14.15.3
lauric acid + NAD(P)H + H+ + O2
-
285363
Pseudomonas oleovorans
12-hydroxydodecanoic acid + NAD(P)+ + H2O
-
-
-
?
1.14.15.3
lecithin + NADH + H+ + O2
-
285363
Pseudomonas oleovorans
?
-
-
-
?
1.14.15.3
methylcyclohexane + NADH + H+ + O2
-
285363
Pseudomonas oleovorans
?
-
-
-
?
1.14.15.3
monoolein + NADH + H+ + O2
-
285363
Pseudomonas oleovorans
18-hydroxyoctadec-9-enoic acid 2,3-dihydroxypropyl ester + NAD+ + H2O
-
-
-
?
1.14.15.3
n-alkane + NAD(P)H + H+ + O2
-
285363
Pseudomonas oleovorans
n-alkanol + NAD(P)+ + H2O
-
-
-
-
1.14.15.3
n-alkane + NAD(P)H + H+ + O2
rubredoxin is electron carrier
285363
Pseudomonas oleovorans
n-alkanol + NAD(P)+ + H2O
-
-
-
?
1.14.15.3
n-alkane + NAD(P)H + H+ + O2
chain length C6-C16, maximal activity with n-octane
285363
Pseudomonas oleovorans
n-alkanol + NAD(P)+ + H2O
-
-
-
?
1.14.15.3
phosphatidylethanolamine + NADH + H+ + O2
-
285363
Pseudomonas oleovorans
?
-
-
-
?
1.14.15.3
phosphatidylserine + NADH + H+ + O2
-
285363
Pseudomonas oleovorans
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.15.3
?
x * 42000, SDS-PAGE
Pseudomonas oleovorans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.15.3
7.5
-
Tris buffer
Pseudomonas oleovorans
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.14.15.3
6.7
7.5
laurate hydroxylation in Tris buffer
Pseudomonas oleovorans