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Literature summary extracted from

  • McKenna, E.J.; Coon, M.J.
    Enzymatic omega-oxidation. IV. Purification and properties of the omega-hydroxylase of Pseudomonas oleovorans (1970), J. Biol. Chem., 245, 3882-3889.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.3 0.032
-
 laurate
-
 Pseudomonas oleovorans
1.14.15.3 0.69
-
 Nonanoate
-
 Pseudomonas oleovorans
1.14.15.3 5.2
-
 Heptanoate
-
 Pseudomonas oleovorans
1.14.15.3 22
-
 hexanoate
-
 Pseudomonas oleovorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.3 Fe non-heme iron protein Pseudomonas oleovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.3 42000
-
 x * 42000, SDS-PAGE Pseudomonas oleovorans
1.14.15.3 2000000
-
 gel filtration Pseudomonas oleovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Pseudomonas oleovorans
-
 n-alkanol + NAD(P)+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.3 Pseudomonas oleovorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.3
-
 Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.3 596
-
-
 Pseudomonas oleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.3 2,5-dimethylhexane + NADH + H+ + O2
-
 Pseudomonas oleovorans ?
-
 ?
1.14.15.3 cyclohexane + NADH + H+ + O2
-
 Pseudomonas oleovorans cyclohexanol + NAD+ + H2O
-
 ?
1.14.15.3 fatty acid + NAD(P)H + H+ + O2 chain length C6-C11, maximal activity with heptanoate Pseudomonas oleovorans omega-hydroxy fatty acid + NAD(P)+ + H2O
-
 ?
1.14.15.3 heptanoate + NADPH + H+ + O2 59% activity compared to octane Pseudomonas oleovorans ?
-
 r
1.14.15.3 hexanoate + NADPH + H+ + O2 25% activity compared to octane Pseudomonas oleovorans ?
-
 r
1.14.15.3 lauric acid + NAD(P)H + H+ + O2
-
 Pseudomonas oleovorans 12-hydroxydodecanoic acid + NAD(P)+ + H2O
-
 ?
1.14.15.3 lecithin + NADH + H+ + O2
-
 Pseudomonas oleovorans ?
-
 ?
1.14.15.3 methylcyclohexane + NADH + H+ + O2
-
 Pseudomonas oleovorans ?
-
 ?
1.14.15.3 monoolein + NADH + H+ + O2
-
 Pseudomonas oleovorans 18-hydroxyoctadec-9-enoic acid 2,3-dihydroxypropyl ester + NAD+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
 Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 rubredoxin is electron carrier Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 chain length C6-C16, maximal activity with n-octane Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 nonanoate + NADPH + H+ + O2 25% activity compared to octane Pseudomonas oleovorans ?
-
 r
1.14.15.3 phosphatidylethanolamine + NADH + H+ + O2
-
 Pseudomonas oleovorans ?
-
 ?
1.14.15.3 phosphatidylserine + NADH + H+ + O2
-
 Pseudomonas oleovorans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.3 ? x * 42000, SDS-PAGE Pseudomonas oleovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.3 7.5
-
 Tris buffer Pseudomonas oleovorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.15.3 6.7 7.5 laurate hydroxylation in Tris buffer Pseudomonas oleovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.3 NADH hydroxylation needs also a NADH-dependent bacterial reductase Pseudomonas oleovorans