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Literature summary extracted from

  • Clemens, S.; Hinderer, W; Wittkampf, U; Barz, W.
    Characterization of cytochrome P450-dependent isoflavone hydroxylases from chickpea (1993), Phytochemistry, 32, 653-657.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.88 BAS110
-
 Cicer arietinum
1.14.14.88 CO ratio CO:O2 of 9:1 leads to 88-96% inhibition Cicer arietinum
1.14.14.88 juglone 0.001 mM juglone reduces activity to 50% Cicer arietinum
1.14.14.88 ketoconazol
-
 Cicer arietinum
1.14.14.88 tetcyclasis
-
 Cicer arietinum
1.14.14.88 Triadimefone
-
 Cicer arietinum
1.14.14.89 BAS 110
-
 Cicer arietinum
1.14.14.89 BAS 111
-
 Cicer arietinum
1.14.14.89 CO ratio CO:O2 of 9:1 leads to 88-96% inhibition Cicer arietinum
1.14.14.89 juglone 0.001 mM juglone reduces activity to 50% Cicer arietinum
1.14.14.89 ketoconazol
-
 Cicer arietinum
1.14.14.89 tetcyclasis
-
 Cicer arietinum
1.14.14.89 Triadimefone
-
 Cicer arietinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.88 11
-
 formononetin
-
 Cicer arietinum
1.14.14.88 12.5
-
 Biochanin A
-
 Cicer arietinum
1.14.14.89 3.3
-
 formononetin
-
 Cicer arietinum
1.14.14.89 13
-
 Biochanin A
-
 Cicer arietinum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.89 Mn2+ cells treated with MnCl2 exhibit 5fold activity after 12 hours Cicer arietinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.88 formononetin + [reduced NADPH-hemoprotein reductase] + O2 Cicer arietinum involved in the biosynthesis of the pterocarpin phytoalexin maackiain, cytochrome P450-dependent monooxygenase 3'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ir
1.14.14.89 formononetin + [reduced NADPH-hemoprotein reductase] + O2 Cicer arietinum involved in the biosynthesis of the pterocarpin phytoalexin medicarpin 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.89 formononetin + [reduced NADPH-hemoprotein reductase] + O2 Cicer arietinum cytochrome P450-dependent monooxygenase 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.88 Cicer arietinum
-
 strain ILC3279 resistant to Ascochyta rabiei and strain ILC susceptible to Ascochyta rabiei, elicitor-induced
-
1.14.14.89 Cicer arietinum
-
 strain ILC3279 resistant to Ascochyta rabiei and strain ILC susceptible to Ascochyta rabiei, elicitor-induced
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.88 biochanin A + [reduced NADPH-hemoprotein reductase] + O2
-
 Cicer arietinum pratensein + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ir
1.14.14.88 formononetin + [reduced NADPH-hemoprotein reductase] + O2
-
 Cicer arietinum 3'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ir
1.14.14.88 formononetin + [reduced NADPH-hemoprotein reductase] + O2 involved in the biosynthesis of the pterocarpin phytoalexin maackiain, cytochrome P450-dependent monooxygenase Cicer arietinum 3'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ir
1.14.14.89 biochanin A + [reduced NADPH-hemoprotein reductase] + O2
-
 Cicer arietinum 2'-hydroxybiochanin A + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.89 formononetin + [reduced NADPH-hemoprotein reductase] + O2 involved in the biosynthesis of the pterocarpin phytoalexin medicarpin Cicer arietinum 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.89 formononetin + [reduced NADPH-hemoprotein reductase] + O2 cytochrome P450-dependent monooxygenase Cicer arietinum 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.89 additional information monohydroxylation of 4'-methoxyisoflavones, no substrates are genistein and daidzein Cicer arietinum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.88 30
-
-
 Cicer arietinum
1.14.14.89 30
-
-
 Cicer arietinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.88 8
-
-
 Cicer arietinum
1.14.14.89 7.4
-
-
 Cicer arietinum