BRENDA - Enzyme Database show

Purification and characterization of cholesterol 7alpha-hydroxylase from rat liver microsomes

Ogishima, T.; Deguchi, S.; Okuda, K.; J. Biol. Chem. 262, 7646-7650 (1987)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.23
additional information
-
additional information
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.23
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.14.23
52000
-
x * 52000, SDS-PAGE
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
Rattus norvegicus
rate-limiting step in bile acid formation
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.23
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.14.23
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.23
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.14.23
156
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.23
5alpha-cholestan-3beta-ol + NADPH + O2
-
285307
Rattus norvegicus
5alpha-cholestan-3beta,7alpha-diol + NADP+ + H2O
-
285307
Rattus norvegicus
?
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation of cholesterol with strict regio- and stereoselectivity, inert towards other sterols and intermediates in conversion of cholesterol to bile acids
285307
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
285307
Rattus norvegicus
?
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
rate-limiting step in bile acid formation
285307
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
285307
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.14.23
?
x * 52000, SDS-PAGE
Rattus norvegicus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.14.23
0.833
-
cholesterol
-
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.23
7.4
-
-
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.23
heme
9 nmol of cytochrome P-450 per mg of protein
Rattus norvegicus
1.14.14.23
NADPH
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.23
heme
9 nmol of cytochrome P-450 per mg of protein
Rattus norvegicus
1.14.14.23
NADPH
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.23
additional information
-
additional information
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.23
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.14.23
52000
-
x * 52000, SDS-PAGE
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
Rattus norvegicus
rate-limiting step in bile acid formation
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.23
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.23
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.14.23
156
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.23
5alpha-cholestan-3beta-ol + NADPH + O2
-
285307
Rattus norvegicus
5alpha-cholestan-3beta,7alpha-diol + NADP+ + H2O
-
285307
Rattus norvegicus
?
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation of cholesterol with strict regio- and stereoselectivity, inert towards other sterols and intermediates in conversion of cholesterol to bile acids
285307
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
285307
Rattus norvegicus
?
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
rate-limiting step in bile acid formation
285307
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
285307
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.14.23
?
x * 52000, SDS-PAGE
Rattus norvegicus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.14.23
0.833
-
cholesterol
-
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.23
7.4
-
-
Rattus norvegicus