BRENDA - Enzyme Database show

Regulation of cholesterol 7alpha-hydroxylase in the liver. Purification of cholesterol 7alpha-hydroxylase and the immunochemical evidence for the induction of cholesterol 7alpha-hydroxylase by cholestyramine and circadian rhythm

Chiang, J.Y.L.; Miller, W.F.; Lin, G.M.; J. Biol. Chem. 265, 3889-3897 (1990)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.14.23
Triton X-100
stimulates
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.23
0.031
-
cholesterol
isoenzyme II
Rattus norvegicus
1.14.14.23
0.052
-
cholesterol
isoenzyme I
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.23
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.14.23
53000
-
x * 53000, SDS-PAGE
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.23
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.14.23
2 isoenzymes: I and II
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.23
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.14.23
36.7
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
285305
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
285305
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.14.23
?
x * 53000, SDS-PAGE
Rattus norvegicus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.14.23
0.0417
-
cholesterol
isoenzyme I
Rattus norvegicus
1.14.14.23
0.0778
-
cholesterol
isoenzyme II
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.23
heme
8 nmol of heme per mg of protein; cytochrome P-450 dependent enzyme
Rattus norvegicus
1.14.14.23
NADPH
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.14.23
Triton X-100
stimulates
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.23
heme
8 nmol of heme per mg of protein; cytochrome P-450 dependent enzyme
Rattus norvegicus
1.14.14.23
NADPH
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.23
0.031
-
cholesterol
isoenzyme II
Rattus norvegicus
1.14.14.23
0.052
-
cholesterol
isoenzyme I
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.23
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.14.23
53000
-
x * 53000, SDS-PAGE
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.23
2 isoenzymes: I and II
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.23
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.14.23
36.7
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
285305
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
285305
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.14.23
?
x * 53000, SDS-PAGE
Rattus norvegicus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.14.23
0.0417
-
cholesterol
isoenzyme I
Rattus norvegicus
1.14.14.23
0.0778
-
cholesterol
isoenzyme II
Rattus norvegicus