BRENDA - Enzyme Database show

Purification of cholesterol 7alpha-hydroxylase from human and rat liver and production of inhibiting polyclonal antibodies

Nguyen, L.B.; Shefer, S.; Salen, G.; Ness, G.; Tanaka, R.D.; Packin, V.; Thomas, P.; Shore, V.; Batta, A.; J. Biol. Chem. 265, 4541-4546 (1990)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.23
microsome
-
Rattus norvegicus
-
-
1.14.14.23
microsome
-
Homo sapiens
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.23
Homo sapiens
-
-
-
1.14.14.23
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.14.23
-
Homo sapiens
1.14.14.23
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.23
liver
-
Rattus norvegicus
-
1.14.14.23
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.14.23
0.00015
-
-
Homo sapiens
1.14.14.23
0.01
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
285302
Homo sapiens
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
285302
Homo sapiens
?
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
285302
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
285302
Rattus norvegicus
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.23
heme
cytochrome P-450 dependent enzyme
Homo sapiens
1.14.14.23
heme
cytochrome P-450 dependent enzyme
Rattus norvegicus
1.14.14.23
NADPH
-
Rattus norvegicus
1.14.14.23
NADPH
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.23
heme
cytochrome P-450 dependent enzyme
Homo sapiens
1.14.14.23
heme
cytochrome P-450 dependent enzyme
Rattus norvegicus
1.14.14.23
NADPH
-
Rattus norvegicus
1.14.14.23
NADPH
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.23
microsome
-
Rattus norvegicus
-
-
1.14.14.23
microsome
-
Homo sapiens
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.23
-
Homo sapiens
1.14.14.23
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.23
liver
-
Rattus norvegicus
-
1.14.14.23
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.14.23
0.00015
-
-
Homo sapiens
1.14.14.23
0.01
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
285302
Homo sapiens
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
285302
Homo sapiens
?
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
285302
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
285302
Rattus norvegicus
?