BRENDA - Enzyme Database show

Cholesterol 7alpha-hydroxylase. 2. Biochemical properties and participation of endogenous cholesterol in the assay in vitro

Van Cantfort, J.; Gielen, J.; Eur. J. Biochem. 55, 33-40 (1975)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.14.23
Thiol-containing substances
e.g. mercaptoethanol, dithiothreitol or cysteamine enhance activity
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.23
Rattus norvegicus
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
285299
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
285299
Rattus norvegicus
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.23
NADPH
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.14.23
Thiol-containing substances
e.g. mercaptoethanol, dithiothreitol or cysteamine enhance activity
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.23
NADPH
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
285299
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
285299
Rattus norvegicus
?