BRENDA - Enzyme Database show

The purification and reconstitution of cytochrome P-450-dependent cholesterol 7alpha-hydroxylase activity from rat liver microsomal fractions

Rush, W.R.; Gibson, G.G.; Parke, D.V.; Biochem. Soc. Trans. 8, 102-103 (1980)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.23
microsome
-
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
Rattus norvegicus
rate-limiting step in bile acid formation
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.23
Rattus norvegicus
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
1.14.14.23
additional information
enzyme has a partial requirement for phospholipid
Rattus norvegicus
Purification (Commentary)
EC Number
Commentary
Organism
1.14.14.23
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.23
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
285298
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
285298
Rattus norvegicus
?
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
rate-limiting step in bile acid formation
285298
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
285298
Rattus norvegicus
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.23
heme
cytochrome P-450 dependent enzyme
Rattus norvegicus
1.14.14.23
NADPH
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.23
heme
cytochrome P-450 dependent enzyme
Rattus norvegicus
1.14.14.23
NADPH
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.23
microsome
-
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
Rattus norvegicus
rate-limiting step in bile acid formation
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Rattus norvegicus
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
1.14.14.23
additional information
enzyme has a partial requirement for phospholipid
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.23
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.23
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
285298
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
285298
Rattus norvegicus
?
1.14.14.23
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
rate-limiting step in bile acid formation
285298
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
285298
Rattus norvegicus
?