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Literature summary extracted from

  • Hurtubise, Y.; Barriault, D.; Sylvestre, M.
    Involvement of the terminal oxygenase beta subunit in the biphenyl dioxygenase reactivity pattern toward chlorobiphenyls (1998), J. Bacteriol., 180, 5828-5835.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.18 in Escherichia coli, strain M15 Pseudomonas sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.18 T375N conversion of sequence to corresponding sequence of Pseudomonas sp. strain LB400 Comamonas testosteroni

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.18 Comamonas testosteroni
-
-
-
1.14.12.18 Pseudomonas sp.
-
-
-
1.14.12.18 Pseudomonas sp. LB400
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.18 2,2'-dichlorobiphenyl + NADH + O2
-
 Pseudomonas sp. 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 2,3-dihydroxy-2'-chlorobiphenyl + NAD+ + HCl
-
 ?
1.14.12.18 2,2'-dichlorobiphenyl + NADH + O2
-
 Comamonas testosteroni 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 2,3-dihydroxy-2'-chlorobiphenyl + NAD+ + HCl
-
 ?
1.14.12.18 2,2'-dichlorobiphenyl + NADH + O2
-
 Pseudomonas sp. LB400 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 2,3-dihydroxy-2'-chlorobiphenyl + NAD+ + HCl
-
 ?
1.14.12.18 2,5,2',5'-tetrachlorobiphenyl + NADH + O2
-
 Pseudomonas sp. cis-3,4-dihydroxy-2,5-dichloro-1-[2',5'-dichlorophenyl]-cyclohexa-1,5-diene + 3,4-dihydroxy-1-phenylcyclohexa-1,5-diene + NAD+ + HCl
-
 ?
1.14.12.18 2,5,2',5'-tetrachlorobiphenyl + NADH + O2
-
 Pseudomonas sp. LB400 cis-3,4-dihydroxy-2,5-dichloro-1-[2',5'-dichlorophenyl]-cyclohexa-1,5-diene + 3,4-dihydroxy-1-phenylcyclohexa-1,5-diene + NAD+ + HCl
-
 ?
1.14.12.18 2,5-dichlorobiphenyl + NADH + O2
-
 Pseudomonas sp. cis-2',3'-dihydroxy-1'-(2,5-dichlorophenyl)-cyclohexa-4',6'-diene + 3,4-dihydroxy-1-phenylcyclohexa-1,5-diene + NAD+ + HCl
-
 ?
1.14.12.18 2,5-dichlorobiphenyl + NADH + O2 no 3,4-dihydrodiol as product Comamonas testosteroni cis-2',3'-dihydroxy-1'-(2,5-dichlorophenyl)-cyclohexa-4',6'-diene + 3,4-dihydroxy-1-phenylcyclohexa-1,5-diene + NAD+ + HCl
-
 ?
1.14.12.18 2,5-dichlorobiphenyl + NADH + O2
-
 Pseudomonas sp. LB400 cis-2',3'-dihydroxy-1'-(2,5-dichlorophenyl)-cyclohexa-4',6'-diene + 3,4-dihydroxy-1-phenylcyclohexa-1,5-diene + NAD+ + HCl
-
 ?
1.14.12.18 3,3'-dichlorobiphenyl + NADH + O2
-
 Pseudomonas sp. 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 4,5-dihydroxy-1-phenylcyclohexa-1,2-diene + NAD+ + HCl
-
 ?
1.14.12.18 3,3'-dichlorobiphenyl + NADH + O2
-
 Comamonas testosteroni 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 4,5-dihydroxy-1-phenylcyclohexa-1,2-diene + NAD+ + HCl
-
 ?
1.14.12.18 3,3'-dichlorobiphenyl + NADH + O2
-
 Pseudomonas sp. LB400 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 4,5-dihydroxy-1-phenylcyclohexa-1,2-diene + NAD+ + HCl
-
 ?
1.14.12.18 biphenyl + NAD(P)H + O2
-
 Comamonas testosteroni 2,3-dihydro-dihydroxybiphenyl + NAD(P)+
-
 ?