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Literature summary extracted from

  • Hurtubise, Y.; Barriault, D.; Sylvestre, M.
    Characterization of active recombinant His-tagged oxygenase component of Comamonas testosteroni B-356 biphenyl dioxygenase (1996), J. Biol. Chem., 271, 8152-8156.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.18 in Escherichia coli, strains M15 and SG13009 Comamonas testosteroni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.12.18 0.094
-
 biphenyl parental protein Comamonas testosteroni
1.14.12.18 1.1
-
 biphenyl recombinant His-tagged protein Comamonas testosteroni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.18 Fe2+ contains a [2Fe-2S] Rieske-type center carrying a mononuclear Fe2+ Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.18 25200
-
 beta subunit with His-tag, SDS-PAGE Comamonas testosteroni
1.14.12.18 44000
-
 native, recombinant and purified alpha subunit with His-tag, HPLC gel filtration Comamonas testosteroni
1.14.12.18 53600
-
 alpha subunit with His-tag, SDS-PAGE Comamonas testosteroni
1.14.12.18 186000
-
 native protein with His-tag, HPLC gel filtration Comamonas testosteroni
1.14.12.18 234000
-
 native protein, HPLC gel filtration Comamonas testosteroni

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.18 Comamonas testosteroni
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.14.12.18 20 min preincubation of His-tagged enzyme with 5 mM dithiothreitol on ice can restore activity of older preparations Comamonas testosteroni

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.18 His-tagged ISP, expressed in Escherichia coli Comamonas testosteroni

Storage Stability

EC Number Storage Stability Organism
1.14.12.18 -70°C, several months Comamonas testosteroni

Subunits

EC Number Subunits Comment Organism
1.14.12.18 heterohexamer alpha3,beta3, 3 * 53600 + 3 * 25200, SDS-PAGE and gel filtration Comamonas testosteroni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.12.18 40
-
-
 Comamonas testosteroni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.12.18 5.5 6
-
 Comamonas testosteroni

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.18 Ferredoxin part of a multicomponent enzyme Comamonas testosteroni