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Literature summary extracted from

  • Roy, G.; Villar, L.M.; Lazaro, I.; Gonzalez, M.; Bootello, A.; Gonzalez-Porque, P.
    Purification and properties of membrane and cytosolic phosphatidylinositol-specific phospholipase C from human spleen (1991), J. Biol. Chem., 266, 11495-11501.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.11 Ca2+ concentrations above 1 mM are inhibitory Homo sapiens
3.1.4.11 EDTA inhibition by EDTA can be reverted upon the addition of increasing concentrations of Ca2+ and Mg2+ in the assay Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.11 cytosol PI-PLCm represents the main activity detected in the membrane, while PI-PLCc is the main activity present in the cytoplasm Homo sapiens 5829
-
3.1.4.11 membrane PI-PLCm represents the main activity detected in the membrane, while PI-PLCc is the main activity present in the cytoplasm Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.11 (NH4)2SO4 high salt concentration, (NH4)2SO4, 2 M, dissociates the high molecular weight form yielding the low molecular form and increasing the specific activity Homo sapiens
3.1.4.11 Ca2+ PIPLC (cytosol) presents a total dependence on the presence of Ca2+ in the assay, which cannot be substituted by other divalent cations (Mg2+, Mn2+, Zn2+) Homo sapiens
3.1.4.11 Mg2+ no requirement for the membrane enzyme solubilized with sodium cholate, activates the membrane enzyme after solubilization in octyl glucoside with maximal activity at 0.1 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.11 additional information
-
PI-PLC, can be resolved into two peaks of activity of high M (60000-70000) and low M (16000-18000) Homo sapiens
3.1.4.11 18000
-
purified Pi-PLC (membrane) has a molecular weight of about 18000, SDS-PAGE or gel filtration Homo sapiens
3.1.4.11 57000
-
purified Pi-PLC (cytosol), SDS-PAGE or gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.11 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.11
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.11 spleen
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.11 1-acyl-2-arachidonoylphosphatidylinositol + H2O
-
Homo sapiens ?
-
?
3.1.4.11 1-acyl-2-linoleoylphosphatidylinositol + H2O
-
Homo sapiens ?
-
?
3.1.4.11 additional information no phospholipase A2 activity is found in the purified enzymes Homo sapiens ?
-
?
3.1.4.11 phosphatidylinositol + H2O Pi-PLC (membrane) and Pi-PLC (cytosol) are specific for this substrate Homo sapiens diacylglycerol + 1D-myo-inositol 1-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.4.11 monomer
-
Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.4.11 low molecular weight PI-PLC
-
Homo sapiens
3.1.4.11 phosphatidylinositol-specific phospholipases C
-
Homo sapiens
3.1.4.11 PI-PLC
-
Homo sapiens
3.1.4.11 PI-PLCc
-
Homo sapiens
3.1.4.11 PI-PLCm
-
Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.11 7
-
Pi-PLC (cytosol) Homo sapiens
3.1.4.11 7
-
Pi-PLC (membrane) solubilized with sodium cholate Homo sapiens
3.1.4.11 7.8
-
assay at Homo sapiens
3.1.4.11 8
-
Pi-PLC (membrane) solubilized with octyl glucoside Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.4.11 additional information
-
both PI-PLC (membrane) and PI-PLC (cytosol) are fairly stable proteins when incubated for short periods of time (30 min) to a wide range of pH. PI-PLC (membrane) shows a fall of about 20-30% activity below pH 5.0 and is stable to alkaline pH up to 11 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.4.11 Homo sapiens purified Pi-PLC (cytosol)
-
6.2
3.1.4.11 Homo sapiens purified Pi-PLC (membrane) 9.2 9