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Literature summary extracted from

  • Allan, D.; Michell, R.H.
    Phosphatidylinositol cleavage catalysed by the soluble fraction from lymphocytes. Activity at pH 5.5 and pH 7.0 (1974), Biochem. J., 142, 591-597.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.11 chlorpromazine stimulates at pH 5.5 Sus scrofa
3.1.4.11 Cinchocaine stimulates at pH 5.5 Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.11 CaCl2 above 0.4 mM, pH 7.0 Sus scrofa
3.1.4.11 chlorpromazine at pH 7.0 Sus scrofa
3.1.4.11 Cinchocaine at pH 7.0 Sus scrofa
3.1.4.11 MgCl2 above 0.4 mM, pH 7.0 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.11 CaCl2 stimulates at pH 5.5, pH dependent Sus scrofa
3.1.4.11 K+ stimulates at pH 5.5 Sus scrofa
3.1.4.11 MgCl2 stimulates at pH 5.5, but less active than CaCl2, pH dependent Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.11 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.11 lymphocyte from mesenteric lymph nodes Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.11 additional information
-
 activity at pH 7.0 (and 0.4 mM CaCl2) is decreased by agents, such as salts and lipophilic cations, which tend to neutralize the negative charge of phosphatidylinositol. At pH 5.5 these agents slightly stimulate activity Sus scrofa
3.1.4.11 0.025
-
 pH 7.3, pellet Sus scrofa
3.1.4.11 0.034
-
 pH 5.4, pellet Sus scrofa
3.1.4.11 0.082
-
 pH 7.3, cell pellet Sus scrofa
3.1.4.11 0.088
-
 pH 5.4, cell pellet Sus scrofa
3.1.4.11 0.26
-
 pH 7.3, supernatant (soluble) Sus scrofa
3.1.4.11 0.28
-
 pH 5.4, supernatant (soluble) Sus scrofa

Storage Stability

EC Number Storage Stability Organism
3.1.4.11 -20°C, stable for several month Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.11 additional information no activity is detectable towards either phosphatidylcholine or phosphatidylethanolamine Sus scrofa ?
-
 ?
3.1.4.11 phosphatidylinositol + H2O
-
 Sus scrofa 1,2-diacylglycerol + 1D-myo-inositol 1-phosphate 1D-myo-inositol 1,2-cyclic phosphate is also formed ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.11 5.4 5.6 5.4-5.6 and 7.0-7.3 Sus scrofa
3.1.4.11 5.6
-
 assay at Sus scrofa
3.1.4.11 7 7.3 5.4-5.6 and 7.0-7.3 Sus scrofa
3.1.4.11 7.1
-
 assay at Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.11 4.5 7.5
-
 Sus scrofa