BRENDA - Enzyme Database

The inhibition of diacylglycerol-stimulated intracellular phospholipases by phospholipids with a phosphocholine-containing polar group. A possible physiological role for sphingomyelin

Dawson, R.M.C.; Hemington, N.; Irvine, R.F.; Biochem. J. 230, 61-68 (1985)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.11
diacylglycerol
the addition of choline phospholipids containing two long hydrophobic chains or choline lysophospholipids containing one long hydrophobic chain produced a total inhibition of the activation which had been produced by diacylglycerol
Homo sapiens
3.1.4.11
diacylglycerol
5-6fold stimulation of rat brain phosphatidylinositol phosphodiesterase. The addition of choline phospholipids containing two long hydrophobic chains or choline lysophospholipids containing one long hydrophobic chain produced a total inhibition of the activation which had been produced by diacylglycerol
Rattus norvegicus
3.1.4.11
phosphatidylethanolamine
activates the hydrolysis. Human platelet phosphatidylinositol phosphodiesterase
Homo sapiens
3.1.4.11
phosphatidylethanolamine
activates the hydrolysis. Human platelet phosphatidylinositol phosphodiesterase
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.11
choline plasmalogen
18.5% of choline plasmalogen is needed for 50% inhibition
Homo sapiens
3.1.4.11
choline plasmalogen
18.5% of choline plasmalogen is needed for 50% inhibition
Rattus norvegicus
3.1.4.11
lysocholine plasmalogen
8.5% of choline plasmalogen is needed for 50% inhibition
Homo sapiens
3.1.4.11
lysocholine plasmalogen
8.5% of choline plasmalogen is needed for 50% inhibition
Rattus norvegicus
3.1.4.11
lysophosphatidylcholine
50% inhibition is produced by 7% molar
Homo sapiens
3.1.4.11
lysophosphatidylcholine
50% inhibition is produced by 7% molar
Rattus norvegicus
3.1.4.11
additional information
enzyme is substantially inhibited by all phospholipids containing a phosphocholine head group. The sphingosine-containing phospholipids are the most inhibitory; phosphatidic acid does not inhibit
Homo sapiens
3.1.4.11
additional information
enzyme is substantially inhibited by all phospholipids containing a phosphocholine head group. The sphingosine-containing phospholipids are the most inhibitory; phosphatidic acid does not inhibit
Rattus norvegicus
3.1.4.11
oleoyl sphingomyelin
-
Homo sapiens
3.1.4.11
oleoyl sphingomyelin
-
Rattus norvegicus
3.1.4.11
phosphatidylcholine
low effect, 50% inhibition is produced by 18% molar egg phosphatidylcholine
Homo sapiens
3.1.4.11
phosphatidylcholine
little effect, 50% inhibition is produced by 18% molar egg phosphatidylcholine
Rattus norvegicus
3.1.4.11
sphingomyelin
50% inhibition at 6.5% molar, and 97% at 20% molar
Homo sapiens
3.1.4.11
sphingomyelin
50% inhibition at low concentration, and 97% at high concentration
Rattus norvegicus
3.1.4.11
sphingosylphosphocholine
-
Homo sapiens
3.1.4.11
sphingosylphosphocholine
-
Rattus norvegicus
3.1.4.11
stearoyl sphingomyelin
-
Homo sapiens
3.1.4.11
stearoyl sphingomyelin
-
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.11
Homo sapiens
-
-
-
3.1.4.11
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.11
blood platelet
-
Homo sapiens
-
3.1.4.11
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.11
phosphatidylinositol + H2O
-
285214
Rattus norvegicus
1D-myo-inositol 1-phosphate
in vitro also inositol cyclic monophosphate formed
-
-
?
3.1.4.11
phosphatidylinositol + H2O
-
285214
Homo sapiens
1D-myo-inositol 1-phosphate
in vitro myo-inositol 1,2-cyclic phosphate also formed
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.11
6.8
-
brain enzyme
Rattus norvegicus
3.1.4.11
7.4
-
platelet enzyme
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.11
diacylglycerol
the addition of choline phospholipids containing two long hydrophobic chains or choline lysophospholipids containing one long hydrophobic chain produced a total inhibition of the activation which had been produced by diacylglycerol
Homo sapiens
3.1.4.11
diacylglycerol
5-6fold stimulation of rat brain phosphatidylinositol phosphodiesterase. The addition of choline phospholipids containing two long hydrophobic chains or choline lysophospholipids containing one long hydrophobic chain produced a total inhibition of the activation which had been produced by diacylglycerol
Rattus norvegicus
3.1.4.11
phosphatidylethanolamine
activates the hydrolysis. Human platelet phosphatidylinositol phosphodiesterase
Homo sapiens
3.1.4.11
phosphatidylethanolamine
activates the hydrolysis. Human platelet phosphatidylinositol phosphodiesterase
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.11
choline plasmalogen
18.5% of choline plasmalogen is needed for 50% inhibition
Homo sapiens
3.1.4.11
choline plasmalogen
18.5% of choline plasmalogen is needed for 50% inhibition
Rattus norvegicus
3.1.4.11
lysocholine plasmalogen
8.5% of choline plasmalogen is needed for 50% inhibition
Homo sapiens
3.1.4.11
lysocholine plasmalogen
8.5% of choline plasmalogen is needed for 50% inhibition
Rattus norvegicus
3.1.4.11
lysophosphatidylcholine
50% inhibition is produced by 7% molar
Homo sapiens
3.1.4.11
lysophosphatidylcholine
50% inhibition is produced by 7% molar
Rattus norvegicus
3.1.4.11
additional information
enzyme is substantially inhibited by all phospholipids containing a phosphocholine head group. The sphingosine-containing phospholipids are the most inhibitory; phosphatidic acid does not inhibit
Homo sapiens
3.1.4.11
additional information
enzyme is substantially inhibited by all phospholipids containing a phosphocholine head group. The sphingosine-containing phospholipids are the most inhibitory; phosphatidic acid does not inhibit
Rattus norvegicus
3.1.4.11
oleoyl sphingomyelin
-
Homo sapiens
3.1.4.11
oleoyl sphingomyelin
-
Rattus norvegicus
3.1.4.11
phosphatidylcholine
low effect, 50% inhibition is produced by 18% molar egg phosphatidylcholine
Homo sapiens
3.1.4.11
phosphatidylcholine
little effect, 50% inhibition is produced by 18% molar egg phosphatidylcholine
Rattus norvegicus
3.1.4.11
sphingomyelin
50% inhibition at 6.5% molar, and 97% at 20% molar
Homo sapiens
3.1.4.11
sphingomyelin
50% inhibition at low concentration, and 97% at high concentration
Rattus norvegicus
3.1.4.11
sphingosylphosphocholine
-
Homo sapiens
3.1.4.11
sphingosylphosphocholine
-
Rattus norvegicus
3.1.4.11
stearoyl sphingomyelin
-
Homo sapiens
3.1.4.11
stearoyl sphingomyelin
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.11
blood platelet
-
Homo sapiens
-
3.1.4.11
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.11
phosphatidylinositol + H2O
-
285214
Rattus norvegicus
1D-myo-inositol 1-phosphate
in vitro also inositol cyclic monophosphate formed
-
-
?
3.1.4.11
phosphatidylinositol + H2O
-
285214
Homo sapiens
1D-myo-inositol 1-phosphate
in vitro myo-inositol 1,2-cyclic phosphate also formed
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.11
6.8
-
brain enzyme
Rattus norvegicus
3.1.4.11
7.4
-
platelet enzyme
Homo sapiens