Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Metz, G.; Röhm.K.H.
    Yeast aminopeptidase I. Chemical composition and catalytic properties (1976), Biochim. Biophys. Acta, 429, 933-949.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.22 2.7
-
 Ala-Thr-Ala methyl ester
-
 Saccharomyces cerevisiae
3.4.11.22 3.2
-
 Ala-Thr-Ala
-
 Saccharomyces cerevisiae
3.4.11.22 6
-
 L-Ala-L-Th
-
 Saccharomyces cerevisiae
3.4.11.22 7
-
 L-Leu-Gly-L-Leu
-
 Saccharomyces cerevisiae
3.4.11.22 12
-
 Ala-Gly-Gly
-
 Saccharomyces cerevisiae
3.4.11.22 14
-
 L-Ala-Gly-Gly ethyl este
-
 Saccharomyces cerevisiae
3.4.11.22 40
-
 Ala-Gly
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.22 Br- activation Saccharomyces cerevisiae
3.4.11.22 Zn2+ activation Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.22 51000
-
 12 * 51000, SDS-PAGE Saccharomyces cerevisiae
3.4.11.22 610000
-
 sucrose density gradient centrifugation Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.22 Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.22 glycoprotein 12% w/w neutral hexoses, 0.5% w/w hexosamines, no sialic acids Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.22
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.22 980
-
 substrate Leu-Gly-Gly Saccharomyces cerevisiae
3.4.11.22 3000
-
 substrate Ala-Thr-Ala Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
3.4.11.22 frozen, 50 mM ammonium acetate buffer, pH 6.2 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.22 L-Ala-Gly + H2O
-
 Saccharomyces cerevisiae L-Ala + Gly
-
 ?
3.4.11.22 L-Ala-Gly-Gly + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.11.22 L-Ala-Gly-Gly ethyl ester + H2O
-
 Saccharomyces cerevisiae L-Ala + Gly-Gly ethyl ester
-
 ?
3.4.11.22 L-Ala-L-Asp-L-Phe methyl ester + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.11.22 L-Ala-L-Leu + H2O
-
 Saccharomyces cerevisiae L-Ala + L-Leu
-
 ?
3.4.11.22 L-Ala-L-Thr + H2O
-
 Saccharomyces cerevisiae L-Ala + L-Thr
-
 ?
3.4.11.22 L-Ala-L-Thr-Gly methyl ester + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.11.22 L-Ala-L-Thr-L-Ala + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.11.22 L-Ala-L-Thr-L-Ala methyl ester + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.11.22 L-Leu-Gly + H2O
-
 Saccharomyces cerevisiae L-Leu + Gly
-
 ?
3.4.11.22 L-Leu-Gly-Gly + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.11.22 L-Leu-Gly-L-Leu + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.11.22 L-Leu-L-Ala + H2O
-
 Saccharomyces cerevisiae L-Leu + L-Ala
-
 ?
3.4.11.22 L-Leu-L-Leu + H2O
-
 Saccharomyces cerevisiae L-Leu + L-Leu
-
 ?
3.4.11.22 L-Phe-Gly + H2O
-
 Saccharomyces cerevisiae L-Phe + Gly
-
 ?
3.4.11.22 L-Phe-Gly-Gly + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.11.22 additional information overview, peptidyl substrates, Leu and other hydrophobic amino acids are best substrates, Gly and other charged amino acids are split off more slowly Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.22 dodecamer 12 * 51000, SDS-PAGE Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.22 33300
-
 Ala-Thr-Ala
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.22 6 6.5 absence of activators Saccharomyces cerevisiae
3.4.11.22 7 8.5 presence of Zn2+ and Cl- Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.22 5 11 4°C, 3 weeks, 10% loss of activity Saccharomyces cerevisiae