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Literature summary extracted from

  • Shinabarger, D.; Berry, A.; May, T.B.; Rothmel, R.; Fialho, A.; Chakrabarty, A.M.
    Purification and characterization of phosphomannose isomerase-guanosine diphospho-D-mannose pyrophosphorylase. A bifunctional enzyme in the alginate biosynthetic pathway of Pseudomonas aeruginosa (1991), J. Biol. Chem., 266, 2080-2088.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.13
-
 Pseudomonas aeruginosa
5.3.1.8
-
 Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.8 additional information point mutation at nucleotide 961 greatly decreases enzyme activity Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.13 additional information not inhibited by dithiothreitol up to 5 mM Pseudomonas aeruginosa
5.3.1.8 dithiothreitol
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.13 0.0142
-
 GDPmannose pH 7.0, 25°C Pseudomonas aeruginosa
2.7.7.13 0.0205
-
 alpha-D-mannose 1-phosphate pH 8.0, 25°C Pseudomonas aeruginosa
2.7.7.13 0.0295
-
 GTP pH 8.0, 25°C Pseudomonas aeruginosa
5.3.1.8 3.03
-
 mannose 6-phosphate bifunctional enzyme: phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.13 Mg2+ Mg2+ or Mn2+ required for activity Pseudomonas aeruginosa
2.7.7.13 Mn2+ Mg2+ or Mn2+ required for activity Pseudomonas aeruginosa
5.3.1.8 Ca2+ activates Pseudomonas aeruginosa
5.3.1.8 Co2+ best activator Pseudomonas aeruginosa
5.3.1.8 Mg2+ activates Pseudomonas aeruginosa
5.3.1.8 Mn2+ activates Pseudomonas aeruginosa
5.3.1.8 additional information activated by bivalent cations in the order: Co2+, Ni2+, Mn2+, Mg2+, Ca2+, Zn2+ Pseudomonas aeruginosa
5.3.1.8 Ni2+ activates Pseudomonas aeruginosa
5.3.1.8 Zinc weak activation Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.13 54000
-
 gel filtration, phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase is a bifunctional enzyme catalyzing both the phosphomannose isomerase, i.e. PIM, and guanosine 5'-diphospho-D-mannose pyrophosphorylase, i.e. GMP, reaction Pseudomonas aeruginosa
2.7.7.13 56000
-
 1 * 56000, SDS-PAGE Pseudomonas aeruginosa
5.3.1.8 54000
-
 bifunctional enzyme: phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase, gel filtration Pseudomonas aeruginosa
5.3.1.8 56000
-
 1 * 56000, bifunctional enzyme: phosphomannose isomerase-guanosine 5-diphospho-D-mannose pyrophosphorylase, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate Pseudomonas aeruginosa enzyme of alginate biosynthetic pathway GDPmannose + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.13 Pseudomonas aeruginosa P07874 phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase is a bifunctional enzyme catalyzing both phosphomannose isomerase, i.e. PIM, and guanosine 5'-diphospho-D-mannose pyrophosphorylase, i.e. GMP, reactions
-
5.3.1.8 Pseudomonas aeruginosa
-
 bifunctional enzyme: phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.13 Biogel, Q-Sepharose, Sephacryl-200 Pseudomonas aeruginosa
5.3.1.8
-
 Pseudomonas aeruginosa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.13 GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose enzyme has both phosphomannose isomerase, i.e. PIM, and guanosine 5'-diphospho-D-mannose pyrophosphorylase, i.e. GMP, activity Pseudomonas aeruginosa
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.8 additional information
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate no activity with GDP-D-glucose, ADP-D-mannose and UDP-D-mannose Pseudomonas aeruginosa GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate enzyme of alginate biosynthetic pathway Pseudomonas aeruginosa GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Pseudomonas aeruginosa diphosphate + GDP-mannose
-
 ?
5.3.1.8 D-Mannose 6-phosphate
-
 Pseudomonas aeruginosa D-Fructose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.13 monomer 1 * 56000, SDS-PAGE Pseudomonas aeruginosa
5.3.1.8 monomer 1 * 56000, bifunctional enzyme: phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
2.7.7.13 PIM-GMP
-
-

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.13 37
-
 assay at Pseudomonas aeruginosa