EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.13 | expression of wild-type and several PMI-GMP mutants in algA mutant 8853 | Pseudomonas aeruginosa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.13 | K175E | approx. 9% of wild-type activity, 600fold increase in Km for mannose 1-phosphate | Pseudomonas aeruginosa |
2.7.7.13 | K175Q | approx. 40% of wild-type activity, 3200fold increase in Km for mannose 1-phosphate | Pseudomonas aeruginosa |
2.7.7.13 | K175R | 470fold increase in mannose 1-phosphate Km value | Pseudomonas aeruginosa |
2.7.7.13 | K20Q | enzyme is unable to support alginate synthesis although it shows no significant differences in Vmax and Km as compared to wild-type | Pseudomonas aeruginosa |
2.7.7.13 | R19H | approx. 50% of wild-type activity, 8fold increase in Km for mannose 1-phosphate | Pseudomonas aeruginosa |
2.7.7.13 | R19K | approx. 50% of wild-type activity, 2 and 6fold increase in Km for mannose 1-phosphate and GTP, respectively | Pseudomonas aeruginosa |
2.7.7.13 | R19L | approx. 50% of wild-type activity, 5fold increase in Km for mannose 1-phosphate and GTP, respectively | Pseudomonas aeruginosa |
2.7.7.13 | S12A | approx. 44% of wild-type activity, 2 and 3fold decrease in Km for mannose 1-phosphate and GTP, respectively | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.13 | 0.0082 | - |
alpha-D-mannose 1-phosphate | 25°C, cosubstrate GTP | Pseudomonas aeruginosa | |
2.7.7.13 | 0.041 | - |
GTP | 25°C, cosubstrate mannose 1-phosphate | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.13 | GTP + alpha-D-mannose 1-phosphate | Pseudomonas aeruginosa | - |
GDPmannose + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.13 | Pseudomonas aeruginosa | P07874 | enzyme is encoded by the algA gene | - |
5.3.1.8 | Pseudomonas aeruginosa | - |
bifunctional enzyme: phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.13 | - |
Pseudomonas aeruginosa |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.7.13 | GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose | PMI and GMP activities are most probably located in catalytically distinct domains | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.13 | GTP + alpha-D-mannose 1-phosphate | - |
Pseudomonas aeruginosa | GDPmannose + diphosphate | - |
? | |
2.7.7.13 | GTP + alpha-D-mannose 1-phosphate | - |
Pseudomonas aeruginosa | diphosphate + GDP-mannose | - |
? | |
5.3.1.8 | D-Mannose 6-phosphate | - |
Pseudomonas aeruginosa | D-Fructose 6-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.1.8 | More | bifunctional enzyme: phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase is composed of two independent enzymatic domains. The carboxyl terminus is critical for mannose-6-phosphate isomerase | Pseudomonas aeruginosa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.13 | PIM-GMP | - |
- |