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Literature summary extracted from

  • Wells, T.N.C.; Scully, P.; Paravicini, G.; Proudfoot, A.E.I.; Payton, M.A.
    Mechanism of irreversible inactivation of phosphomannose isomerase by silver ions and flamazine (1995), Biochemistry, 34, 7896-7903.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.8 C150A mutant Cys150Ala shows similar Km-values and maximal velocity as compared to the wild-type enzyme. The mutant enzyme shows no inhibition by silver sulfadiazine, and is 1000fold less sensitive to Hg2+ inhibition Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.8 Ag+ irreversible inhibition in a two-step process, mannose 6-phosphate protects against inactivation. Mutant enzyme Cys150Ala shows 1000fold less sensitivity than the wild-type enzyme Candida albicans
5.3.1.8 Silver sulfadiazine wild-type enzyme is inhibited, mutant enzyme Cys150Ala is not inhibited Candida albicans
5.3.1.8 Silver sulfadiazine no inhibition Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.8 additional information Candida albicans enzyme is involved in biosynthesis of mannan components of the cell walls ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.8 Candida albicans
-
-
-
5.3.1.8 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.8 D-Mannose 6-phosphate
-
 Escherichia coli D-Fructose 6-phosphate
-
 ?
5.3.1.8 D-Mannose 6-phosphate
-
 Candida albicans D-Fructose 6-phosphate
-
 ?
5.3.1.8 additional information enzyme is involved in biosynthesis of mannan components of the cell walls Candida albicans ?
-
 ?