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Literature summary extracted from
- The effect of temperature on ribose-5-phosphate isomerase from mesophile Thiobacillus thioparus, and a thermophile, Bacillus caldolyticus (1976), J. Biochem., 79, 1331-1344.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.6 | 2-mercaptoethanol | stimulates | Thiobacillus thioparus |  |
| 5.3.1.6 | 2-mercaptoethanol | stimulates | [Bacillus] caldolyticus | |
| 5.3.1.6 | EDTA | stimulates | Thiobacillus thioparus | |
| 5.3.1.6 | EDTA | stimulates | [Bacillus] caldolyticus | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 5.3.1.6 | (NH4)2SO4, NaCl, KCl and LiCl increase thermal stability. LiBr, CaCl2, methanol, ethanol, and 1-propanol decrease thermal stability. Alcohols decrease the stability in the following order: methanol, ethanol, propanol | Thiobacillus thioparus |
| 5.3.1.6 | (NH4)2SO4, NaCl, KCl and LiCl increase thermal stability. LiBr, CaCl2, methanol, ethanol, and 1-propanol decrease thermal stability. Alcohols decrease the stability in the following order: methanol, ethanol, propanol | [Bacillus] caldolyticus |
| 5.3.1.6 | ethylene glycol has little effect on the mesophilic enzyme | Thiobacillus thioparus |
| 5.3.1.6 | ethylene glycol increases stability | [Bacillus] caldolyticus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.6 | 6-phosphogluconate | temperature-dependent inhibition | [Bacillus] caldolyticus | |
| 5.3.1.6 | 6-phosphogluconate | temperature-dependent inhibition | Thiobacillus thioparus | |
| 5.3.1.6 | Ag+ | 1 mM | [Bacillus] caldolyticus | |
| 5.3.1.6 | Ag+ | 1 mM | Thiobacillus thioparus | |
| 5.3.1.6 | AMP | temperature-dependent inhibition | [Bacillus] caldolyticus | |
| 5.3.1.6 | AMP | temperature-dependent inhibition | Thiobacillus thioparus | |
| 5.3.1.6 | Co2+ | 1 mM | [Bacillus] caldolyticus | |
| 5.3.1.6 | Co2+ | 1 mM | Thiobacillus thioparus | |
| 5.3.1.6 | Mg2+ | 0.01 M | [Bacillus] caldolyticus | |
| 5.3.1.6 | Mg2+ | 0.01 M | Thiobacillus thioparus | |
| 5.3.1.6 | Phenylmercuriacetate | reversible by 0.02-10 mM 2-mercaptoethanol, and by 10 mM Cys | [Bacillus] caldolyticus | |
| 5.3.1.6 | Phenylmercuriacetate | reversible by 0.02-10 mM 2-mercaptoethanol, and by 10 mM Cys | Thiobacillus thioparus | |
| 5.3.1.6 | Sodium mersalyl | reversible by 2-mercaptoethanol, 0.02-10 mM, and by Cys, 10 mM | [Bacillus] caldolyticus | |
| 5.3.1.6 | Sodium mersalyl | reversible by 2-mercaptoethanol, 0.02-10 mM, and by Cys, 10 mM | Thiobacillus thioparus | |
| 5.3.1.6 | Zn2+ | 0.01 M | [Bacillus] caldolyticus | |
| 5.3.1.6 | Zn2+ | 0.01 M | Thiobacillus thioparus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.6 | 40000 | - |
gel filtration | Thiobacillus thioparus |
| 5.3.1.6 | 40000 | - |
gel filtration | [Bacillus] caldolyticus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.6 | Thiobacillus thioparus | - |
- |
- |
| 5.3.1.6 | [Bacillus] caldolyticus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.6 | - |
Thiobacillus thioparus |
| 5.3.1.6 | - |
[Bacillus] caldolyticus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.6 | 52.7 | - |
- |
[Bacillus] caldolyticus |
| 5.3.1.6 | 544 | - |
- |
Thiobacillus thioparus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.6 | D-ribose 5-phosphate | - |
Thiobacillus thioparus | D-ribulose 5-phosphate | - |
? | |
| 5.3.1.6 | D-ribose 5-phosphate | - |
[Bacillus] caldolyticus | D-ribulose 5-phosphate | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.6 | 45 | - |
pH 5-10, stable for at least 30 min | Thiobacillus thioparus |
| 5.3.1.6 | 57 | - |
1 h, 50% loss of activity | Thiobacillus thioparus |
| 5.3.1.6 | 65 | - |
pH 5-10, stable for at least 30 min | [Bacillus] caldolyticus |
| 5.3.1.6 | 91 | - |
1 h, 50% loss of activity | [Bacillus] caldolyticus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.6 | 6.5 | 9 | - |
Thiobacillus thioparus |
| 5.3.1.6 | 6.5 | 9 | - |
[Bacillus] caldolyticus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.6 | 5 | 10 | 65°C, stable for at least 30 min | [Bacillus] caldolyticus |
| 5.3.1.6 | 5 | 10 | 45°C, stable for at least 30 min | Thiobacillus thioparus |
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