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Literature summary extracted from

  • Van Tillbeurgh, H.; Jenkins, J.; Chiadmi, M.; Janin, J.; Wodak, S.J.; Mrabet, N.T.; Lambeir, A.M.
    Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis. 3. Changing metal specificity and the pH profile by site-directed mutagenesis (1992), Biochemistry, 31, 5467-5471.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.5 E186D mutant enzymes E186D and E186Q are active, and their metal specificity is different from that of the wild type. The E186 enzyme is most active with Mn2+ and has a drastically shifted pH optimum Actinoplanes missouriensis
5.3.1.5 E186Q mutant enzymes E186D and E186Q are active, and their metal specificity is different from that of the wild type. The E186 enzyme is most active with Mn2+ and has a drastically shifted pH optimum Actinoplanes missouriensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 additional information
-
 additional information Km-values for wild-type enzyme and mutants E186D and E186Q, activated by Mg2+, Mn2+ or Co2+ Actinoplanes missouriensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.5 Co2+ Mg2+, Mn2+ or Co2+ required for maximal activity Actinoplanes missouriensis
5.3.1.5 Mg2+
-
 Actinoplanes missouriensis
5.3.1.5 Mn2+
-
 Actinoplanes missouriensis

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Actinoplanes missouriensis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Xylose
-
 Actinoplanes missouriensis D-Xylulose
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.5 additional information
-
 additional information turnover numbers for wild-type enzyme and mutant enzymes E186D and E186Q, activated by Mg2+, Mn2+ or Co2+ Actinoplanes missouriensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.5 6.25
-
 mutant E186Q, xylose isomerization, activated by Mn2+ or Mg2+ Actinoplanes missouriensis
5.3.1.5 7.3
-
 wild-type enzyme, xylose isomerization, activated by Mg2+ Actinoplanes missouriensis
5.3.1.5 7.9
-
 wild-type enzyme, xylose isomerization, activated by Mg2+ Actinoplanes missouriensis