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Literature summary extracted from

  • Lee, C.; Bagdasarian, M.; Meng, M.; Zeikus, J.G.
    Catalytic mechanism of xylose (glucose) isomerase from Clostridium thermosulfurogenes. Characterization of the structural gene and function of active site histidine (1990), J. Biol. Chem., 265, 19082-19090.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.5 expression in Escherichia coli, sequence homology with enzymes from other sources Thermoanaerobacterium thermosulfurigenes

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.5 H101F substitution of His101 by Phe completely abolishes enzyme activity. When His101 is changed to Glu, Gln, Asn, or Asp, approximately 10-16% of wild-type enzyme activity is retained by the mutant enzymes. The His101Gln mutant enzyme is resistant to diethyldicarbonate inhibition which completely inactivates the wild-type enzyme Thermoanaerobacterium thermosulfurigenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 130
-
 D-glucose D-glucose, mutant enzyme His71Phe Thermoanaerobacterium thermosulfurigenes
5.3.1.5 138
-
 D-glucose mutant enzyme His101Gln Thermoanaerobacterium thermosulfurigenes
5.3.1.5 142
-
 D-glucose glucose, wild-type enzyme Thermoanaerobacterium thermosulfurigenes
5.3.1.5 152
-
 D-glucose mutant enzyme His152Phe Thermoanaerobacterium thermosulfurigenes
5.3.1.5 168
-
 D-glucose mutant enzyme His101Asn Thermoanaerobacterium thermosulfurigenes
5.3.1.5 198
-
 D-glucose mutant enzyme His101Asp Thermoanaerobacterium thermosulfurigenes
5.3.1.5 252
-
 D-glucose mutant enzyme His101Glu Thermoanaerobacterium thermosulfurigenes

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Thermoanaerobacterium thermosulfurigenes
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.5 additional information
-
-
 Thermoanaerobacterium thermosulfurigenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Glucose
-
 Thermoanaerobacterium thermosulfurigenes D-Fructose
-
 ?
5.3.1.5 D-Xylose
-
 Thermoanaerobacterium thermosulfurigenes D-Xylulose
-
 ?