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Literature summary extracted from

  • Charlier, J.; Gerlo, E.
    Arginyl-tRNA synthetase from Escherichia coli K12. Purification, properties, and sequence of substrate addition (1979), Biochemistry, 18, 3171-3178.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.1.1.19 affected by repeated freeze-thaw cycles Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.19 8-azido-ATP
-
 Escherichia coli
6.1.1.19 homoarginine
-
 Escherichia coli
6.1.1.19 p-hydroxymercuribenzoate tRNA or DTT protects against heat inactivation Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.19 60000
-
 1 * 60000, SDS-PAGE Escherichia coli
6.1.1.19 63000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.19 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.19
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.19 ATP + L-arginine + tRNAArg = AMP + diphosphate + L-arginyl-tRNAArg random addition of substrates with all steps in rapid equilibrium except for the interconversion of the central quarternary complexes Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.19 4.984
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
6.1.1.19 -20°C, 50% v/v glycerol, stable for at least 6 months Escherichia coli

Subunits

EC Number Subunits Comment Organism
6.1.1.19 monomer 1 * 60000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.19 8.1 8.5
-
 Escherichia coli