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Literature summary extracted from

  • Rangarajan, M.; Hartley, B.S.
    Mechanism of D-fructose isomerization by Arthrobacter D-xylose isomerase (1992), Biochem. J., 283, 223-233.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.5 5-Thio-alpha-D-glucopyranose competitive Arthrobacter sp.
5.3.1.5 Ca2+ strict competitive against Mg2+ Arthrobacter sp.
5.3.1.5 D-sorbitol competitive Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 3.3
-
D-xylose D-xylose, with 30 mM Mg2+ Arthrobacter sp.
5.3.1.5 110
-
D-fructose with 10-400 mM Mg2+ Arthrobacter sp.
5.3.1.5 230
-
D-glucose
-
Arthrobacter sp.
5.3.1.5 800
-
D-fructose with 1-10 mM Mg2+ Arthrobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.5 Co2+ less effective than Mg2+ in D-fructose isomerization Arthrobacter sp.
5.3.1.5 Mg2+ best activator of D-fructose isomerization Arthrobacter sp.
5.3.1.5 Mn2+ less effective than Mg2+ in activation of D-fructose isomerization Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Arthrobacter sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Fructose compulsory order of binding: 1. Co2+ binds first to site 2 and then to site 1, then the D-fructose binds at site 1. At normal concentrations Mg2+ binds at site 1, then D-fructose, and then Mg2+ at site 2. At very high Mg2+ concentrations, above 10 mM, the order is Mg2+ at site1, Mg2+ at site 2, then D-fructose Arthrobacter sp. ?
-
?
5.3.1.5 D-Glucose
-
Arthrobacter sp. D-Fructose
-
?
5.3.1.5 D-Xylose
-
Arthrobacter sp. D-Xylulose
-
?