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Literature summary extracted from

  • Chauthaiwale, J.; Rao, M.
    Production and purification of extracellular D-xylose isomerase from an alkaliphilic, thermophilic Bacillus sp. (1994), Appl. Environ. Microbiol., 60, 4495-4499.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.5 EDTA complete inactivation in presence of Mg2+, Co2+ or Mn2+ Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 6.66
-
 D-xylose
-
 Bacillus sp. (in: Bacteria)
5.3.1.5 142
-
 D-glucose
-
 Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.1.5 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.5 Co2+ when the metal ions are added separately the activation is 48-52%. The combination of Mg2+ and Co2+ results in activation of 83%. The combination of Mg2+ and Mn2+ or Co2+ and Mn2+ results in 58-59% activation Bacillus sp. (in: Bacteria)
5.3.1.5 Co2+ Mg2+, Mn2+ or Co2+ required for maximal activity Bacillus sp. (in: Bacteria)
5.3.1.5 Mg2+ when the metal ions are added separately the activation is 48-52%. The combination of Mg2+ and Co2+ results in activation of 83%. The combination of Mg2+ and Mn2+ or Co2+ and Mn2+ results in 58-59% activation Bacillus sp. (in: Bacteria)
5.3.1.5 Mg2+ Mg2+, Mn2+ or Co2+ required for maximal activity Bacillus sp. (in: Bacteria)
5.3.1.5 Mn2+ when the metal ions are added separately the activation is 48-52%. The combination of Mg2+ and Co2+ results in activation of 83%. The combination of Mg2+ and Mn2+ or Co2+ and Mn2+ results in 58-59% activation Bacillus sp. (in: Bacteria)
5.3.1.5 Mn2+ Mg2+, Mn2+ or Co2+ required for maximal activity Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.5 50000
-
 3 * 50000, SDS-PAGE Bacillus sp. (in: Bacteria)
5.3.1.5 160000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.5
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.5 16.4
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Glucose
-
 Bacillus sp. (in: Bacteria) D-Fructose
-
 r
5.3.1.5 D-Xylose
-
 Bacillus sp. (in: Bacteria) D-Xylulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.5 trimer 3 * 50000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.5 85
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.5 50 80 30 min, in presence of Co2+, stable Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.5 50
-
 D-glucose
-
 Bacillus sp. (in: Bacteria)
5.3.1.5 230
-
 D-xylose
-
 Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.5 8
-
-
 Bacillus sp. (in: Bacteria)