Literature summary extracted from

Hess, J.M.; Tchernajenko, V.; Vieille, C.; Zeikus, J.G.; Kelly, R.M.
Thermotoga neapolitana homotetrameric xylose isomerase is expressed as a catalytically active and thermostable dimer in Escherichia coli (1998), Appl. Environ. Microbiol., 64, 2357-2360.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
5.3.1.5	expression in Escherichia coli	Thermotoga neapolitana

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
5.3.1.5	50000	-	2 * 50000, the recombinant enzyme exists as homodimer and homotetramer, with the dimer being the more abundant form. The native enzyme exclusively exists as tetramer	Thermotoga neapolitana
5.3.1.5	50000	-	4 * 50000, the recombinant enzyme exists as homodimer and homotetramer, with the dimer being the more abundant form. The native enzyme exclusively exists as tetramer	Thermotoga neapolitana
5.3.1.5	210000	-	gel filtration	Thermotoga neapolitana

Organism

EC Number	Organism	UniProt	Comment	Textmining
5.3.1.5	Thermotoga neapolitana	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
5.3.1.5	-	Thermotoga neapolitana

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5.3.1.5	D-Xylose	-	Thermotoga neapolitana	D-Xylulose	-	?

Subunits

EC Number	Subunits	Comment	Organism
5.3.1.5	dimer	2 * 50000, the recombinant enzyme exists as homodimer and homotetramer, with the dimer being the more abundant form. The native enzyme exclusively exists as tetramer	Thermotoga neapolitana
5.3.1.5	tetramer	4 * 50000, the recombinant enzyme exists as homodimer and homotetramer, with the dimer being the more abundant form. The native enzyme exclusively exists as tetramer	Thermotoga neapolitana

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
5.3.1.5	95	-	-	Thermotoga neapolitana

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.3.1.5	7	-	-	Thermotoga neapolitana

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Release 2023.1 (January 2023)