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Literature summary extracted from

  • Kawai, Y.; Konishi, H.; Horitsu, H.; Sakurai, H.; Takamizawa, K.; Suzuki, T.; Kawai, K.
    Purification and characterization of D-xylose isomerase from Bifidobacterium adolescentis (1994), Biosci. Biotechnol. Biochem., 58, 691-694.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 4
-
 D-xylose
-
 Bifidobacterium adolescentis
5.3.1.5 298
-
 D-glucose
-
 Bifidobacterium adolescentis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.5 53000
-
 3 * 53000, SDS-PAGE Bifidobacterium adolescentis
5.3.1.5 168000
-
 gel filtration Bifidobacterium adolescentis

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Bifidobacterium adolescentis
-
-
-
5.3.1.5 Streptomyces phaeochromogenes
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
5.3.1.5 no glycoprotein
-
 Bifidobacterium adolescentis

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.5
-
 Bifidobacterium adolescentis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.5 3.17
-
-
 Bifidobacterium adolescentis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Glucose
-
 Bifidobacterium adolescentis D-Fructose
-
 r
5.3.1.5 D-Xylose
-
 Bifidobacterium adolescentis D-Xylulose
-
 ?
5.3.1.5 D-Xylose
-
 Streptomyces phaeochromogenes D-Xylulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.5 trimer 3 * 53000, SDS-PAGE Bifidobacterium adolescentis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.5 60
-
-
 Bifidobacterium adolescentis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.3.1.5 30 70 30°C: about 45% of maximal activity, 70°C: about 10% of maximal activity Bifidobacterium adolescentis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.5 40
-
 10 min, stable up to Bifidobacterium adolescentis
5.3.1.5 60
-
 10 min, 20% loss of activity Bifidobacterium adolescentis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.5 7
-
-
 Bifidobacterium adolescentis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.5 6 9 pH 6: about 70% of maximal activity, pH 9: about 90% of maximal activity Bifidobacterium adolescentis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.5 6
-
 8°C, 18 h, about 50% loss of activity Bifidobacterium adolescentis
5.3.1.5 7 8 8°C, 18 h, stable Bifidobacterium adolescentis
5.3.1.5 9
-
 8°C, 18 h, about 10% loss of activity Bifidobacterium adolescentis