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Literature summary extracted from

  • Lehmbacher, A.; Bisswanger, H.
    Isolation and characterization of an extremely thermostable D-xylose isomerase from Thermus aquaticus HB8 (1990), J. Gen. Microbiol., 136, 679-686.
No PubMed abstract available

General Stability

EC Number General Stability Organism
5.3.1.5 immobilized enzyme remains stable for at least 45 d at 4°C or at 25°C, upon incubation at 70°C the half-life is about 4 d Thermus aquaticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.5 50000
-
 4 * 50000, SDS-PAGE Thermus aquaticus
5.3.1.5 196000
-
 gel filtration Thermus aquaticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.5 additional information Thermus aquaticus important enzyme in degradation of xylans and metabolizing D-xylose via the pentose phosphate pathway ?
-
 ?
5.3.1.5 additional information Thermus aquaticus HB8 important enzyme in degradation of xylans and metabolizing D-xylose via the pentose phosphate pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Thermus aquaticus
-
-
-
5.3.1.5 Thermus aquaticus HB8
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.5
-
 Thermus aquaticus

Storage Stability

EC Number Storage Stability Organism
5.3.1.5 4°C, stable for more than 1 month Thermus aquaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Glucose
-
 Thermus aquaticus D-Fructose
-
 ?
5.3.1.5 D-Glucose
-
 Thermus aquaticus HB8 D-Fructose
-
 ?
5.3.1.5 D-Xylose
-
 Thermus aquaticus D-Xylulose
-
 ?
5.3.1.5 D-Xylose
-
 Thermus aquaticus HB8 D-Xylulose
-
 ?
5.3.1.5 additional information important enzyme in degradation of xylans and metabolizing D-xylose via the pentose phosphate pathway Thermus aquaticus ?
-
 ?
5.3.1.5 additional information important enzyme in degradation of xylans and metabolizing D-xylose via the pentose phosphate pathway Thermus aquaticus HB8 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.5 tetramer 4 * 50000, SDS-PAGE Thermus aquaticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.5 25
-
 stable for more than 1 month Thermus aquaticus
5.3.1.5 70
-
 half-life in absence of divalent cations: 4 d. In presence of Mn2+ or Co2+ stable for at least 1 month Thermus aquaticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.5 5.5 8.5
-
 Thermus aquaticus