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Literature summary extracted from

  • Inyang, C.U.; Gebhart, U.; Obi, S.K.C.; Bisswanger, H.
    Isolation and characterization of a D-glucose/xylose isomerase from a new thermophilic strain Streptomyces sp. (PLC) (1995), Appl. Microbiol. Biotechnol., 43, 632-638.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.5 Cu2+
-
 Streptomyces sp.
5.3.1.5 D-sorbitol
-
 Streptomyces sp.
5.3.1.5 D-xylitol competitive Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 35
-
 D-xylose
-
 Streptomyces sp.
5.3.1.5 400
-
 D-glucose
-
 Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.5 Co2+ two metal ions bind per subunit to non-identical sites. Mg2+, Mn2+, and Co2+ are of comparable efficiency for the D-xylose isomerization. Co2+ is the most efficient cofactor for D-glucose isomerization Streptomyces sp.
5.3.1.5 Mg2+ two metal ions bind per subunit to non-identical sites. Mg2+, Mn2+, and Co2+ are of comparable efficiency for the D-xylose isomerization Streptomyces sp.
5.3.1.5 Mn2+ two metal ions bind per subunit to non-identical sites. Mg2+, Mn2+, and Co2+ are of comparable efficiency for the D-xylose isomerization Streptomyces sp.
5.3.1.5 Zn2+ weak cofactor for D-glucose isomerization Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.5 183000
-
 sucrose density gradient centrifugation Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Streptomyces sp.
-
 thermophilic strain PLC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.5
-
 Streptomyces sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.5 6.4
-
-
 Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Glucose
-
 Streptomyces sp. D-Fructose
-
 ?
5.3.1.5 D-Xylose
-
 Streptomyces sp. D-Xylulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.5 tetramer
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.5 78
-
 D-glucose, activity increases up to 85°C and remains constant nearly to 100°C Streptomyces sp.
5.3.1.5 85
-
 D-xylose,activity increases up to 85°C and remains constant nearly to 100°C Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.5 53
-
 half life in presence of Co2+: 7 days, half-life in presence of Mg2+: 9 days, in presence of Mn2+ the enzyme activity remains constant for at least 10 days Streptomyces sp.
5.3.1.5 70
-
 in presence of Mn2+, 50% loss of activity after 5 days Streptomyces sp.
5.3.1.5 98
-
 half-life: 15 min Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.5 7
-
-
 Streptomyces sp.