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Literature summary extracted from

  • Callens, M.; Kersters-Hilderson, H.; van Opstal, O.; de Bruyne, C.K.
    Catalytic properties of D-xylose isomerase from Streptomyces violaceoruber (1986), Enzyme Microb. Technol., 8, 696-700.
No PubMed abstract available

General Stability

EC Number General Stability Organism
5.3.1.5 Co2+ is superior as protector against thermal inactivation at 80°C Streptomyces violaceoruber

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.5 D-sorbitol
-
 Streptomyces violaceoruber
5.3.1.5 D-xylitol
-
 Streptomyces violaceoruber
5.3.1.5 Tris(hydroxymethyl)aminomethane
-
 Streptomyces violaceoruber

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 2.9
-
 D-xylose with 10 mM Mg2+ Streptomyces violaceoruber
5.3.1.5 3.3
-
 D-xylose with 1 mM Co2+ Streptomyces violaceoruber

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.5 Co2+ activates Streptomyces violaceoruber
5.3.1.5 Fe2+ slight activation Streptomyces violaceoruber
5.3.1.5 Mg2+ activates, maximal activity at 10 mM Streptomyces violaceoruber
5.3.1.5 Mn2+ activates Streptomyces violaceoruber

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Streptomyces violaceoruber
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Glucose
-
 Streptomyces violaceoruber D-Fructose
-
 ?
5.3.1.5 D-Xylose r Streptomyces violaceoruber D-Xylulose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.5 80
-
-
 Streptomyces violaceoruber

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.5 additional information
-
 Co2+ is superior as protector against thermal inactivation at 80°C Streptomyces violaceoruber
5.3.1.5 60
-
 30 days, stable in presence of 1 mM Co2+ or 10 mM Mg2+ Streptomyces violaceoruber

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.5 2.8
-
 D-xylose with 1 mM Co2+ Streptomyces violaceoruber
5.3.1.5 4.15
-
 D-xylose with 10 mM Mg2+ Streptomyces violaceoruber

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.5 7.5 9.5
-
 Streptomyces violaceoruber