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Literature summary extracted from
- Physico-chemical and enzymatic properties of purified glucose isomerases from Streptomyces olivochromogenes and Bacillus stearothermophilus (1978), Agric. Biol. Chem., 42, 909-917.
No PubMed abstract available
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.3.1.5 | - |
Streptomyces olivochromogenes |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.5 | Cu2+ | - |
Geobacillus stearothermophilus |  |
| 5.3.1.5 | Cu2+ | - |
Streptomyces olivochromogenes | |
| 5.3.1.5 | Mn2+ | - |
Streptomyces olivochromogenes | |
| 5.3.1.5 | Ni2+ | - |
Geobacillus stearothermophilus | |
| 5.3.1.5 | Ni2+ | - |
Streptomyces olivochromogenes | |
| 5.3.1.5 | Sn2+ | - |
Geobacillus stearothermophilus | |
| 5.3.1.5 | Zn2+ | - |
Geobacillus stearothermophilus | |
| 5.3.1.5 | Zn2+ | - |
Streptomyces olivochromogenes | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.5 | 18 | - |
xylulose | - |
Streptomyces olivochromogenes | |
| 5.3.1.5 | 33 | - |
D-xylose | - |
Streptomyces olivochromogenes | |
| 5.3.1.5 | 100 | - |
D-xylulose | - |
Geobacillus stearothermophilus | |
| 5.3.1.5 | 200 | - |
D-fructose | - |
Geobacillus stearothermophilus | |
| 5.3.1.5 | 200 | - |
D-fructose | - |
Streptomyces olivochromogenes | |
| 5.3.1.5 | 220 | - |
D-fructose | - |
Streptomyces olivochromogenes | |
| 5.3.1.5 | 250 | - |
D-glucose | - |
Streptomyces olivochromogenes | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.5 | 56000 | - |
2 * 56000, SDS-PAGE | Streptomyces olivochromogenes |
| 5.3.1.5 | 115000 | 117000 | low speed sedimentation without reaching equilibrium, equilibrium sedimentation | Streptomyces olivochromogenes |
| 5.3.1.5 | 127000 | - |
equilibrium sedimentation | Geobacillus stearothermophilus |
| 5.3.1.5 | 130000 | - |
1 * 130000, SDS-PAGE | Geobacillus stearothermophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.5 | Geobacillus stearothermophilus | - |
- |
- |
| 5.3.1.5 | Streptomyces olivochromogenes | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.5 | - |
Geobacillus stearothermophilus |
| 5.3.1.5 | - |
Streptomyces olivochromogenes |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.5 | 2.4 | - |
- |
Streptomyces olivochromogenes |
| 5.3.1.5 | 2.7 | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.5 | D-Fructose | - |
Geobacillus stearothermophilus | ? | - |
? | |
| 5.3.1.5 | D-Fructose | - |
Streptomyces olivochromogenes | ? | - |
? | |
| 5.3.1.5 | D-Glucose | - |
Geobacillus stearothermophilus | D-Fructose | - |
? | |
| 5.3.1.5 | D-Glucose | - |
Streptomyces olivochromogenes | D-Fructose | - |
? | |
| 5.3.1.5 | D-Xylose | - |
Geobacillus stearothermophilus | D-Xylulose | - |
? | |
| 5.3.1.5 | D-Xylose | r | Streptomyces olivochromogenes | D-Xylulose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.1.5 | dimer | 2 * 56000, SDS-PAGE | Streptomyces olivochromogenes |
| 5.3.1.5 | monomer | 1 * 130000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.5 | 80 | - |
- |
Geobacillus stearothermophilus |
| 5.3.1.5 | 80 | - |
- |
Streptomyces olivochromogenes |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.5 | 50 | - |
30 min, stable | Streptomyces olivochromogenes |
| 5.3.1.5 | 65 | - |
30 min, about 50% loss of activity | Streptomyces olivochromogenes |
| 5.3.1.5 | 70 | - |
- |
Geobacillus stearothermophilus |
| 5.3.1.5 | 70 | - |
30 min, about 70% loss of activity | Streptomyces olivochromogenes |
| 5.3.1.5 | 75 | - |
60 min, stable up to | Geobacillus stearothermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.5 | 7.5 | 8 | - |
Geobacillus stearothermophilus |
| 5.3.1.5 | 8 | 10 | - |
Streptomyces olivochromogenes |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.5 | 6 | 10 | pH 6: about 35% of maximal activity, pH 10: about 30% of maximal activity | Streptomyces olivochromogenes |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.5 | 4 | - |
room temperature, 30 min, stable above | Streptomyces olivochromogenes |
| 5.3.1.5 | 5 | - |
room temperature, 30 min, stable above | Geobacillus stearothermophilus |
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