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Literature summary extracted from

  • Schulz, M.; Weissenböck, G.
    Partial purification and characterization of luteolin-triglucuronide-specific beta-glucuronidase from rye primary leaves (Secale cereale) (1987), Phytochemistry, 26, 933-937.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.31 ethyleneglycol activation Secale cereale

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.31 Ag+
-
 Secale cereale
3.2.1.31 Cu2+
-
 Secale cereale
3.2.1.31 D-saccharic acid 1,4-lactone
-
 Secale cereale
3.2.1.31 methanol
-
 Secale cereale

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.31 0.007
-
 luteolin 7-O-diglucuronide 4'-O-glucuronide
-
 Secale cereale

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.31 luteolin triglucuronide + H2O Secale cereale
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.31 Secale cereale
-
 rye
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.31 partial Secale cereale

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.31 leaf
-
 Secale cereale
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.31 additional information
-
-
 Secale cereale

Storage Stability

EC Number Storage Stability Organism
3.2.1.31 -20°C, 50% glycerol, at least 2 months Secale cereale

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.31 apigenin 7,4'-diglucuronide + H2O
-
 Secale cereale apigenin 7-O-glucuronide + glucuronic acid
-
 ?
3.2.1.31 luteolin 7-O-glucuronide + H2O
-
 Secale cereale ?
-
 ?
3.2.1.31 luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] + H2O
-
 Secale cereale ?
-
 ?
3.2.1.31 luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]-4'-O-glucuronide + H2O
-
 Secale cereale ?
-
 ?
3.2.1.31 luteolin triglucuronide + H2O
-
 Secale cereale ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.31 55
-
-
 Secale cereale

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.31 55
-
 denaturation above Secale cereale

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.31 4.3
-
-
 Secale cereale

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.31 2.1 5.8 half maximal activity Secale cereale