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Literature summary extracted from

  • Anderson, R.L.; Allison, D.P.
    Purification and characterization of D-lyxose isomerase (1965), J. Biol. Chem., 240, 2367-2372.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.15 EDTA
-
 Klebsiella aerogenes
5.3.1.15 PCMB
-
 Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.15 3.6
-
 D-Lyxose
-
 Klebsiella aerogenes
5.3.1.15 10
-
 mannose
-
 Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.15 Ca2+ metal ion required, Ca2+ activates slightly Klebsiella aerogenes
5.3.1.15 Co2+ metal ion required, Co2+ activates slightly Klebsiella aerogenes
5.3.1.15 Fe2+ metal ion required, Fe2+ activates Klebsiella aerogenes
5.3.1.15 Fe3+ metal ion required, Fe3+ activates Klebsiella aerogenes
5.3.1.15 Mn2+ metal ion required, Mn2+ activates most effectively. Km: 0.0004 mM Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.15 40000
-
 gel filtration, density gradient centrifugation Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.15 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.15
-
 Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.15 D-Lyxose
-
 Klebsiella aerogenes D-Xylulose
-
 ?
5.3.1.15 D-Mannose
-
 Klebsiella aerogenes D-Fructose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.15 7
-
-
 Klebsiella aerogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.15 4.9 7 pH 4.0: about 50% of maximal activity, pH 7.0: maximal activity Klebsiella aerogenes