Any feedback?
Literature summary extracted from
- Triose-phosphate isomerase of Leishmania mexicana mexicana. Cloning and characterization of the gene, overexpression in Escherichia coli and analysis of the protein (1994), Eur. J. Biochem., 220, 331-338.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.1 | overexpression in Escherichia coli | Leishmania mexicana |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.1 | 0.3 | - |
D-glyceraldehyde 3-phosphate | recombinant enzyme | Leishmania mexicana |  |
| 5.3.1.1 | 0.31 | - |
D-glyceraldehyde 3-phosphate | - |
Leishmania mexicana | |
| 5.3.1.1 | 1.1 | - |
glycerone phosphate | glycerone phosphate | Leishmania mexicana | |
| 5.3.1.1 | 1.3 | - |
glycerone phosphate | recombinant enzyme | Leishmania mexicana | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.3.1.1 | glycosome | - |
Leishmania mexicana | 20015 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.1 | 28000 | - |
2 * 28000, SDS-PAGE | Leishmania mexicana |
| 5.3.1.1 | 44800 | - |
recombinant enzyme, gel filtration | Leishmania mexicana |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.1 | Leishmania mexicana | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.1 | - |
Leishmania mexicana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.3.1.1 | promastigote | - |
Leishmania mexicana | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.1 | additional information | - |
- |
Leishmania mexicana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.1 | D-Glyceraldehyde 3-phosphate | r | Leishmania mexicana | Glycerone phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.1.1 | dimer | 2 * 28000, SDS-PAGE | Leishmania mexicana |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.1 | 42 | - |
stable for at least 30 min | Leishmania mexicana |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.1 | 367 | - |
glycerone phosphate | - |
Leishmania mexicana | |
| 5.3.1.1 | 4000 | - |
D-glyceraldehyde 3-phosphate | - |
Leishmania mexicana | |
| 5.3.1.1 | 4170 | - |
D-glyceraldehyde 3-phosphate | recombinant enzyme | Leishmania mexicana | |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.1 | 6.5 | 8.5 | 20 h, stable | Leishmania mexicana |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
