Literature summary extracted from

Borchert, T.V.; Pratt, K.; Zeelen, J.P.; Callens, M.; Noble, M.E.M.; Opperdoes, F.R.; Michels, P.A.M.; Wierenga, R.K.
Overexpression of trypanosomal triosephosphate isomerase in Escherichia coli and characterisation of a dimer-interface mutant (1993), Eur. J. Biochem., 211, 703-710.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
5.3.1.1	expression in Escherichia coli	Trypanosoma brucei

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
5.3.1.1	-	Trypanosoma brucei

Protein Variants

EC Number	Protein Variants	Comment	Organism
5.3.1.1	H47N	mutant His47Asn, dimer is considerably less stable than wild-type trypanosomal enzyme	Trypanosoma brucei

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
5.3.1.1	0.19	-	D-glyceraldehyde 3-phosphate	recombinant wild-type enzyme	Trypanosoma brucei
5.3.1.1	0.25	-	D-glyceraldehyde 3-phosphate	D-glyceraldehyde 3-phosphate	Trypanosoma brucei
5.3.1.1	1.1	-	glycerone phosphate	recombinant wild-type enzyme	Trypanosoma brucei
5.3.1.1	1.2	-	glycerone phosphate	-	Trypanosoma brucei

Organism

EC Number	Organism	UniProt	Comment	Textmining
5.3.1.1	Trypanosoma brucei	-	-	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.3.1.1	2500	-	recombinant wild-type enzyme	Trypanosoma brucei

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5.3.1.1	D-Glyceraldehyde 3-phosphate	r	Trypanosoma brucei	Glycerone phosphate	-	?

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
5.3.1.1	1000	-	glycerone phosphate	recombinant enzyme	Trypanosoma brucei
5.3.1.1	1080	-	glycerone phosphate	-	Trypanosoma brucei
5.3.1.1	6000	-	D-glyceraldehyde 3-phosphate	recombinant enzyme	Trypanosoma brucei
5.3.1.1	6170	-	D-glyceraldehyde 3-phosphate	-	Trypanosoma brucei

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Release 2023.1 (January 2023)