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Literature summary extracted from

  • Misset, O.; Opperdoes, F.R.
    Simultaneous purification of hexokinase, class-I fructose-bisphosphate aldolase, triosephosphate isomerase and phosphoglycerate kinase from Trypanosoma brucei (1984), Eur. J. Biochem., 144, 475-483.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.1 pharmacology hexokinase may be an excellent target for the development of a rational chemotherapy against the parasite Trypanosoma brucei

General Stability

EC Number General Stability Organism
2.7.2.3 extensive dialysis against 0.1 M Tris/HCl, pH 7.6, 1 mM DTT, 1 mM EDTA, 1 mM sodium azide, 0.2 M ammonium sulfate, stable Trypanosoma brucei
2.7.2.3 freezing inactivates Trypanosoma brucei
2.7.2.3 stable as a suspension in ammonium sulfate, 70-90% saturation Trypanosoma brucei
4.1.2.13 freezing inactivates Trypanosoma brucei
5.3.1.1 no loss of activity when dialyzed extensively against 0.1 M Tris/HCl, pH 7.6, containing 1 mM dithiothreitol, 1 mM EDTA, 1 mM sodium azide and 0.2 M ammonium sulfate Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.13 EDTA insensitive Trypanosoma brucei
4.1.2.13 NaBH4 in presence of substrate Trypanosoma brucei
4.1.2.13 Zn2+ ZnSO4 Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.1 glycosome
-
 Trypanosoma brucei 20015
-
2.7.2.3 glycosome
-
 Trypanosoma brucei 20015
-
5.3.1.1 glycosome
-
 Trypanosoma brucei 20015
-
5.3.1.9 glycosome
-
 Trypanosoma brucei 20015
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.3 Mg2+
-
 Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 50000
-
 x * 50000, SDS-PAGE Trypanosoma brucei
2.7.2.3 46000
-
 x * 46000, SDS-PAGE Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.1 D-glucose + ATP Trypanosoma brucei rate-limiting step in the glycolysis D-glucose 6-phosphate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Trypanosoma brucei
-
 bloodstream forms, stock 427
-
2.7.2.3 Trypanosoma brucei
-
 bloodstream form, stock 427
-
4.1.2.13 Trypanosoma brucei
-
-
-
5.3.1.1 Trypanosoma brucei
-
-
-
5.3.1.9 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 260fold Trypanosoma brucei
2.7.2.3 660fold, to homogeneity Trypanosoma brucei
4.1.2.13 simultaneous purification of EC 2.7.1.1, EC 4.1.2.13, EC 5.3.1.1, and EC 2.7.2.3 Trypanosoma brucei
5.3.1.1
-
 Trypanosoma brucei
5.3.1.9 partial Trypanosoma brucei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 5.6
-
 in purified glycosomes, at 25°C, pH 7.6 Trypanosoma brucei
2.7.1.1 190
-
 pure enzyme, at 25°C, pH 7.6 Trypanosoma brucei
2.7.2.3 504
-
 purified enzyme Trypanosoma brucei
4.1.2.13 additional information
-
-
 Trypanosoma brucei
5.3.1.1 additional information
-
-
 Trypanosoma brucei

Storage Stability

EC Number Storage Stability Organism
2.7.1.1 4°C, extensive dialysis of purified enzyme against 0.1 M Tris/HCl, pH 7.6, 1 mM dithiothreitol, 1 mM EDTA, 1 mM sodium azide, 0.2 M ammonium sulfate, stable Trypanosoma brucei
4.1.2.13 4°C, no loss of activity after 2 months Trypanosoma brucei
5.3.1.1 stable upon prolonged storage in 0.1 M Tris/HCl buffer, pH 7.6, containing 1 mM dithiothreitol, 1 mM EDTA, 1 mM sodium azide and 0.2 M ammonium sulfate Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 D-glucose + ATP
-
 Trypanosoma brucei ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP rate-limiting step in the glycolysis Trypanosoma brucei D-glucose 6-phosphate + ADP
-
 ?
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Trypanosoma brucei ADP + 1,3-diphosphoglycerate
-
 r
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Trypanosoma brucei glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.13 D-Fructose 1-phosphate at 15% of the activity with fructose 1,6-diphosphate Trypanosoma brucei Glycerone phosphate + D-glyceraldehyde
-
 ?
5.3.1.1 D-Glyceraldehyde 3-phosphate
-
 Trypanosoma brucei Glycerone phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.1 ? x * 50000, SDS-PAGE Trypanosoma brucei
2.7.2.3 ? x * 46000, SDS-PAGE Trypanosoma brucei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.1 25
-
 assay at Trypanosoma brucei
2.7.2.3 25
-
 assay at Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 7.6
-
 assay at Trypanosoma brucei
2.7.2.3 7.6
-
 assay at Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP
-
 Trypanosoma brucei
2.7.2.3 ADP
-
 Trypanosoma brucei
2.7.2.3 ATP required as phosphate donor Trypanosoma brucei