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Literature summary extracted from

  • Ogata, K.; Ohno, R.; Morioka, S.; Terao, K.
    Further study on association of 5SrRNA-L5 protein complex and methionyl-tRNA to methionyl-tRNA synthetase in the macromolecular aminoacyl-tRNA synthetase complex (1996), J. Biochem., 120, 869-880.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.10 5SrRNA positive effector of MetRS, markedly enhances the MetRS activity in the MetRS complex Rattus norvegicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.11.1 crystallization in presence of ZnSO4, NaCl, and 2-methyl-2,4-pentanediol, at pH 7.8 in Tris buffer, X-ray diffraction crystal structure determination and analysis at 1.6 A resolution Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 amastatin natural inhibitor Bos taurus
3.4.11.1 Amino acid hydroxamates
-
 Bos taurus
3.4.11.1 bestatin natural inhibitor Bos taurus
3.4.11.1 L-leucylthiol
-
 Bos taurus
3.4.11.1 leucinal
-
 Bos taurus
3.4.11.1 leucine phosphonic acid
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 6
-
 L-Leu-4-nitroanilide Zn2+-Zn2+-enzyme form Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 bicarbonate activates Bos taurus
3.4.11.1 Mg2+ activates Bos taurus
3.4.11.1 Mn2+ activates Bos taurus
3.4.11.1 additional information the enzyme contains no disulfide bonds Bos taurus
3.4.11.1 Zn2+ zinc-metallopeptidase, two Zn2+ per protomer, the zinc bound at residue 488 can be exchanged for the less preferable Mn2+, Co2+, or Mg2+, dimetal site structure involving Asp332, Asp255, Asp273, Lys250, and Glu334 for binding of the two catalytic zinc ions, overview Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Bos taurus
-
-
-
6.1.1.10 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.1 no glycoprotein
-
 Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 native enzyme from lens to homogeneity by ammonium sulfate fractionation, and gel filtration, the enzyme shows low solubility under low salt conditions Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 lens
-
 Bos taurus
-
6.1.1.10 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-Leu-4-nitroanilide + H2O
-
 Bos taurus L-Leu + 4-nitroaniline
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Rattus norvegicus AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 hexamer homohexamer, lens enzyme three-dimensional structure and protein fold, overview Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 LAP
-
 Bos taurus
3.4.11.1 leucine aminopeptidase
-
 Bos taurus
3.4.11.1 More the enzyme belongs to the peptidase family M17 Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.1 0.7
-
 L-Leu-4-nitroanilide Zn2+-Zn2+-enzyme form Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.1 0.0000013
-
 amastatin
-
 Bos taurus